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Original Article
Seroprevalence of Toxoplasmosis in Puerperal Women Treated at a Tertiary Referral Hospital
- Juliana Fernandes Medeiros
, - Ana Cláudia Rabelo e Silva ,
- Natália Domene Franco da Rocha ,
- Alexia Viegas Georg ,
- Patricia Pereira dos Santos Melli , [ ... ],
- Geraldo Duarte
07-10-2023
Summary
Original ArticleSeroprevalence of Toxoplasmosis in Puerperal Women Treated at a Tertiary Referral Hospital
Revista Brasileira de Ginecologia e Obstetrícia. 2023;45(2):059-064
07-10-2023- Juliana Fernandes Medeiros ,
- Ana Cláudia Rabelo e Silva ,
- Natália Domene Franco da Rocha ,
- Alexia Viegas Georg ,
- Patricia Pereira dos Santos Melli ,
- Silvana Maria Quintana ,
- Geraldo Duarte
Views164See moreAbstract
Objective
To evaluate the seroprevalence of toxoplasmosis among puerperal women cared for at a tertiary university hospital and the level of understanding of these puerperal women about toxoplasmosis, vertical transmission, and its prophylaxis.
Methods
For this cross-sectional study, we evaluated 225 patients using presential interviews, prenatal documentation, and electronic medical records. Data were stored using Research Electronic Data Capture (REDCap) software. Prevalence rates were estimated by the presence of reactive IgG antibodies against Toxoplasma gondii. Data analysis was performed using the chi-square test and calculation of the odds ratio (OR). Seroreactivity to T. gondii and exposure variables (age, educational level, and parity) were analyzed using a confidence interval (95%CI) and a significance level of 5% (p < 0.05).
Results
The seropositivity rate for T. gondii was 40%. There was no association between seroprevalence and age. Primiparity was a protective factor against seropositivity and low education was a risk factor.
Conclusion
Knowledge of T. gondii infection and its transmission forms was significantly limited, presenting a risk for acute maternal toxoplasmosis and vertical transmission of this protozoan. Increasing the education level regarding the risk of toxoplasmosis during pregnancy could reduce the rates of infection and vertical transmission of this parasite.
Views164
This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Summary
Original ArticleSeroprevalence of Toxoplasmosis in Puerperal Women Treated at a Tertiary Referral Hospital
Revista Brasileira de Ginecologia e Obstetrícia. 2023;45(2):059-064
07-10-2023- Juliana Fernandes Medeiros ,
- Ana Cláudia Rabelo e Silva ,
- Natália Domene Franco da Rocha ,
- Alexia Viegas Georg ,
- Patricia Pereira dos Santos Melli ,
- Silvana Maria Quintana ,
- Geraldo Duarte
Views164See moreAbstract
Objective
To evaluate the seroprevalence of toxoplasmosis among puerperal women cared for at a tertiary university hospital and the level of understanding of these puerperal women about toxoplasmosis, vertical transmission, and its prophylaxis.
Methods
For this cross-sectional study, we evaluated 225 patients using presential interviews, prenatal documentation, and electronic medical records. Data were stored using Research Electronic Data Capture (REDCap) software. Prevalence rates were estimated by the presence of reactive IgG antibodies against Toxoplasma gondii. Data analysis was performed using the chi-square test and calculation of the odds ratio (OR). Seroreactivity to T. gondii and exposure variables (age, educational level, and parity) were analyzed using a confidence interval (95%CI) and a significance level of 5% (p < 0.05).
Results
The seropositivity rate for T. gondii was 40%. There was no association between seroprevalence and age. Primiparity was a protective factor against seropositivity and low education was a risk factor.
Conclusion
Knowledge of T. gondii infection and its transmission forms was significantly limited, presenting a risk for acute maternal toxoplasmosis and vertical transmission of this protozoan. Increasing the education level regarding the risk of toxoplasmosis during pregnancy could reduce the rates of infection and vertical transmission of this parasite.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. 
- Juliana Fernandes Medeiros
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Original Article
IgG Avidity in Samples Collected on Filter Paper: Importance of The Early Diagnosis of Congenital Toxoplasmosis
- Jéssica Yonara de Souza ,
- Taynara Cristina Gomes ,
- Hanstter Hallison Alves Rezende ,
- Heloisa Ribeiro Storchilo ,
- Patrícia Giffron Rodrigues , [ ... ],
- Ana Maria de Castro
01-24-2021
Summary
Original ArticleIgG Avidity in Samples Collected on Filter Paper: Importance of The Early Diagnosis of Congenital Toxoplasmosis
Revista Brasileira de Ginecologia e Obstetrícia. 2021;43(12):887-893
01-24-2021- Jéssica Yonara de Souza ,
- Taynara Cristina Gomes ,
- Hanstter Hallison Alves Rezende ,
- Heloisa Ribeiro Storchilo ,
- Patrícia Giffron Rodrigues ,
- Ana Maria de Castro
Views159See moreAbstract
Objective
The purpose of the present study is to standardize and evaluate the use of the immunoglobulin G (IgG) antibody avidity test on blood samples from newborns collected on filter paper to perform the heel test aiming at its implementation in ongoing programs.
Methods
Blood samples from newborns were collected on filter paper simultaneously with the heel prick test. All samples were subjected to immunoglobulin M IgM and IgG enzyme-linked immunosorbent assays (ELISA). Peripheral blood was collected again in the traditional way and on filter paper from newborns with high IgG levels (33). Three types of techniques were performed, the standard for measuring IgG in serum, adapted for filter paper and the technique of IgG avidity in serum and on filter paper. The results of the avidity test were classified according to the Rahbari protocol.
Results
Among the 177 samples, 17 were collected in duplicate from the same child, 1 of peripheral blood and 1 on filter paper. In this analysis, 1 (5.88%) of the 17 samples collected in duplicate also exhibited low IgG avidity, suggesting congenital infection. In addition, the results obtained from serum and filter paper were in agreement, that is, 16 (94.12%) samples presented high avidity, with 100% agreement between the results obtained from serum and from filter paper.
Conclusion
The results of the present study indicate that the avidity test may be another valuable method for the diagnosis of congenital toxoplasmosis in newborns.
Views159This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Summary
Original ArticleIgG Avidity in Samples Collected on Filter Paper: Importance of The Early Diagnosis of Congenital Toxoplasmosis
Revista Brasileira de Ginecologia e Obstetrícia. 2021;43(12):887-893
01-24-2021- Jéssica Yonara de Souza ,
- Taynara Cristina Gomes ,
- Hanstter Hallison Alves Rezende ,
- Heloisa Ribeiro Storchilo ,
- Patrícia Giffron Rodrigues ,
- Ana Maria de Castro
Views159See moreAbstract
Objective
The purpose of the present study is to standardize and evaluate the use of the immunoglobulin G (IgG) antibody avidity test on blood samples from newborns collected on filter paper to perform the heel test aiming at its implementation in ongoing programs.
Methods
Blood samples from newborns were collected on filter paper simultaneously with the heel prick test. All samples were subjected to immunoglobulin M IgM and IgG enzyme-linked immunosorbent assays (ELISA). Peripheral blood was collected again in the traditional way and on filter paper from newborns with high IgG levels (33). Three types of techniques were performed, the standard for measuring IgG in serum, adapted for filter paper and the technique of IgG avidity in serum and on filter paper. The results of the avidity test were classified according to the Rahbari protocol.
Results
Among the 177 samples, 17 were collected in duplicate from the same child, 1 of peripheral blood and 1 on filter paper. In this analysis, 1 (5.88%) of the 17 samples collected in duplicate also exhibited low IgG avidity, suggesting congenital infection. In addition, the results obtained from serum and filter paper were in agreement, that is, 16 (94.12%) samples presented high avidity, with 100% agreement between the results obtained from serum and from filter paper.
Conclusion
The results of the present study indicate that the avidity test may be another valuable method for the diagnosis of congenital toxoplasmosis in newborns.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. - Jéssica Yonara de Souza
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Original Article
Comparison between Enzyme Immunoassays Performed on Samples of Dried Blood and Serum for Toxoplasmosis Prenatal Screening: Population-based Study
- Bárbara Araújo Marques ,
- Ericka Vianna Machado Carellos ,
- Vânia Maria Novato Silva ,
- Fernando Henrique Pereira ,
- Maria Regina Lage Guerra , [ ... ],
- Gláucia Manzan Queiroz de Andrade
07-30-2021
Summary
Original ArticleComparison between Enzyme Immunoassays Performed on Samples of Dried Blood and Serum for Toxoplasmosis Prenatal Screening: Population-based Study
Revista Brasileira de Ginecologia e Obstetrícia. 2021;43(5):351-356
07-30-2021- Bárbara Araújo Marques ,
- Ericka Vianna Machado Carellos ,
- Vânia Maria Novato Silva ,
- Fernando Henrique Pereira ,
- Maria Regina Lage Guerra ,
- Jacqueline Araújo Domingos Iturra ,
- José Nélio Januário ,
- Gláucia Manzan Queiroz de Andrade
Views152Abstract
Objective
Most prenatal screening programs for toxoplasmosis use immunoassays in serum samples of pregnant women. Few studies assess the accuracy of screening tests in dried blood spots, which are of easy collection, storage, and transportation. The goals of the present study are to determine the performance and evaluate the agreement between an immunoassay of dried blood spots and a reference test in the serum of pregnant women from a population-based prenatal screening program for toxoplasmosis in Brazil.
Methods
A cross-sectional study was performed to compare the immunoassays Imunoscreen Toxoplasmose IgM and Imunoscreen Toxoplasmose IgG (Mbiolog Diagnósticos, Ltda., Contagem, Minas Gerais, Brazil)in dried blood spots with the enzymelinked fluorescent assay (ELFA, BioMérieux S.A., Lyon, France) reference standard in the serum of pregnant women from Minas Gerais Congenital Toxoplasmosis Control Program.
Results
The dried blood spot test was able to discriminate positive and negative results of pregnant women when comparedwith the reference test, with an accuracy of 98.2% for immunoglobulin G (IgG), and of 95.8% for immunoglobulin M (IgM).
Conclusion
Dried blood samples are easy to collect, store, and transport, and they have a good performance,making this a promisingmethod for prenatal toxoplasmosis screening programs in countries with continental dimensions, limited resources, and a high prevalence of toxoplasmosis, as is the case of Brazil.
Key-words Congenital toxoplasmosisdried blood spot testingPrenatal careprenatal diagnosisToxoplasmosisSee moreViews152This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Summary
Original ArticleComparison between Enzyme Immunoassays Performed on Samples of Dried Blood and Serum for Toxoplasmosis Prenatal Screening: Population-based Study
Revista Brasileira de Ginecologia e Obstetrícia. 2021;43(5):351-356
07-30-2021- Bárbara Araújo Marques ,
- Ericka Vianna Machado Carellos ,
- Vânia Maria Novato Silva ,
- Fernando Henrique Pereira ,
- Maria Regina Lage Guerra ,
- Jacqueline Araújo Domingos Iturra ,
- José Nélio Januário ,
- Gláucia Manzan Queiroz de Andrade
Views152Abstract
Objective
Most prenatal screening programs for toxoplasmosis use immunoassays in serum samples of pregnant women. Few studies assess the accuracy of screening tests in dried blood spots, which are of easy collection, storage, and transportation. The goals of the present study are to determine the performance and evaluate the agreement between an immunoassay of dried blood spots and a reference test in the serum of pregnant women from a population-based prenatal screening program for toxoplasmosis in Brazil.
Methods
A cross-sectional study was performed to compare the immunoassays Imunoscreen Toxoplasmose IgM and Imunoscreen Toxoplasmose IgG (Mbiolog Diagnósticos, Ltda., Contagem, Minas Gerais, Brazil)in dried blood spots with the enzymelinked fluorescent assay (ELFA, BioMérieux S.A., Lyon, France) reference standard in the serum of pregnant women from Minas Gerais Congenital Toxoplasmosis Control Program.
Results
The dried blood spot test was able to discriminate positive and negative results of pregnant women when comparedwith the reference test, with an accuracy of 98.2% for immunoglobulin G (IgG), and of 95.8% for immunoglobulin M (IgM).
Conclusion
Dried blood samples are easy to collect, store, and transport, and they have a good performance,making this a promisingmethod for prenatal toxoplasmosis screening programs in countries with continental dimensions, limited resources, and a high prevalence of toxoplasmosis, as is the case of Brazil.
Key-words Congenital toxoplasmosisdried blood spot testingPrenatal careprenatal diagnosisToxoplasmosisSee moreThis is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. - Bárbara Araújo Marques
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Original Article
Follow-up of Toxoplasmosis during Pregnancy: Ten-Year Experience in a University Hospital in Southern Brazil
- Amanda Andrade Diesel,
- Suzana de Azevedo Zachia,
- Ana Lúcia Letti Müller ,
- Amanda Vilaverde Perez,
- Flavio Antonio de Freitas Uberti, [ ... ],
- José Antônio de Azevedo Magalhães
09-30-2019
Summary
Original ArticleFollow-up of Toxoplasmosis during Pregnancy: Ten-Year Experience in a University Hospital in Southern Brazil
Revista Brasileira de Ginecologia e Obstetrícia. 2019;41(9):539-547
09-30-2019- Amanda Andrade Diesel,
- Suzana de Azevedo Zachia,
- Ana Lúcia Letti Müller ,
- Amanda Vilaverde Perez,
- Flavio Antonio de Freitas Uberti,
- José Antônio de Azevedo Magalhães
Views170See moreAbstract
Objective
To describe a population of pregnant women diagnosed with toxoplasmosis and their respective newborns, describing the hospital protocol for treatment and follow-up.
Methods
Retrospective cohort of pregnant women with acute toxoplasmosis infection and risk of transplacental transmission who were sent to the Fetal Medicine Group of Hospital de Clínicas de Porto Alegre (HCPA) between - January 1, 2006 and December 31, 2016. All patients with confirmed disease were included. The diagnostic protocol and treatment were applied; a polymerase chain reaction (PCR) analysis of the amniotic fluid was used to diagnose toxoplasmosis and determine the treatment. The newborns were followed up at the pediatric outpatient clinic specializing in congenital infection. The patients who were not followed up or were not born in the HCPA were excluded.
Results
A total of 65 patients were confirmed to have gestational toxoplasmosis; 40 performed amniocentesis, and 6 (15%) were identified as having positive PCR in the amniotic fluid. In five of those cases, this result associated with the gestational age defined the triple therapy during pregnancy, and in one case, it defined the monotherapy (advanced gestational age). A total of 4 of these newborns were treated from birth with triple therapy for 10months, 1 was not treated (due to maternal refusal), and 1 progressed to death within the first 54 hours of life due to complications of congenital toxoplasmosis. Of the 34 remaining cases with a negative PCR, 33 were treated with monotherapy and 1 was treated with triple therapy (ultrasound findings); of these children, 9 (26.5%) presented negative immunoglobulin G (IgG), 24 (70.6%) presented positive IgG (but none presented positive immunoglobulin M [IgM]), and 1 (2,9%) presented alterations compatible with congenital disease and started treatment with the triple therapy soon after birth. Out of the total sample of 60 patients, among the 25 who did not perform amniotic fluid PCR, 5 were treated with triple therapy (ultrasound findings/prior treatment) and 20 patients were submitted to monotherapy; only two newborns underwent treatment for congenital toxoplasmosis. Among the 65 cases of gestational toxoplasmosis, 6 (9,2%) children had a diagnosis of congenital toxoplasmosis, and 2 patients with triple therapy felt severe adverse effects of the medications.
Conclusions
The present study suggests that research on PCR screening of the amniotic fluid may be useful to identify patients with a higher potential for fetal complications, who may benefit from the poly-antimicrobial treatment. Patients with negative PCR results must continue to prevent fetal infection with monotherapy, without risk of fetal or maternal impairment.
Views170This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Summary
Original ArticleFollow-up of Toxoplasmosis during Pregnancy: Ten-Year Experience in a University Hospital in Southern Brazil
Revista Brasileira de Ginecologia e Obstetrícia. 2019;41(9):539-547
09-30-2019- Amanda Andrade Diesel,
- Suzana de Azevedo Zachia,
- Ana Lúcia Letti Müller ,
- Amanda Vilaverde Perez,
- Flavio Antonio de Freitas Uberti,
- José Antônio de Azevedo Magalhães
Views170See moreAbstract
Objective
To describe a population of pregnant women diagnosed with toxoplasmosis and their respective newborns, describing the hospital protocol for treatment and follow-up.
Methods
Retrospective cohort of pregnant women with acute toxoplasmosis infection and risk of transplacental transmission who were sent to the Fetal Medicine Group of Hospital de Clínicas de Porto Alegre (HCPA) between - January 1, 2006 and December 31, 2016. All patients with confirmed disease were included. The diagnostic protocol and treatment were applied; a polymerase chain reaction (PCR) analysis of the amniotic fluid was used to diagnose toxoplasmosis and determine the treatment. The newborns were followed up at the pediatric outpatient clinic specializing in congenital infection. The patients who were not followed up or were not born in the HCPA were excluded.
Results
A total of 65 patients were confirmed to have gestational toxoplasmosis; 40 performed amniocentesis, and 6 (15%) were identified as having positive PCR in the amniotic fluid. In five of those cases, this result associated with the gestational age defined the triple therapy during pregnancy, and in one case, it defined the monotherapy (advanced gestational age). A total of 4 of these newborns were treated from birth with triple therapy for 10months, 1 was not treated (due to maternal refusal), and 1 progressed to death within the first 54 hours of life due to complications of congenital toxoplasmosis. Of the 34 remaining cases with a negative PCR, 33 were treated with monotherapy and 1 was treated with triple therapy (ultrasound findings); of these children, 9 (26.5%) presented negative immunoglobulin G (IgG), 24 (70.6%) presented positive IgG (but none presented positive immunoglobulin M [IgM]), and 1 (2,9%) presented alterations compatible with congenital disease and started treatment with the triple therapy soon after birth. Out of the total sample of 60 patients, among the 25 who did not perform amniotic fluid PCR, 5 were treated with triple therapy (ultrasound findings/prior treatment) and 20 patients were submitted to monotherapy; only two newborns underwent treatment for congenital toxoplasmosis. Among the 65 cases of gestational toxoplasmosis, 6 (9,2%) children had a diagnosis of congenital toxoplasmosis, and 2 patients with triple therapy felt severe adverse effects of the medications.
Conclusions
The present study suggests that research on PCR screening of the amniotic fluid may be useful to identify patients with a higher potential for fetal complications, who may benefit from the poly-antimicrobial treatment. Patients with negative PCR results must continue to prevent fetal infection with monotherapy, without risk of fetal or maternal impairment.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. 
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Original Articles
Evaluation of Pregnant and Postpartum Women’s Knowledge about Toxoplasmosis in Rio Grande – RS, Brazil
Revista Brasileira de Ginecologia e Obstetrícia. 2016;38(11):538-544
11-01-2016
Summary
Original ArticlesEvaluation of Pregnant and Postpartum Women’s Knowledge about Toxoplasmosis in Rio Grande – RS, Brazil
Revista Brasileira de Ginecologia e Obstetrícia. 2016;38(11):538-544
11-01-2016Views114See moreAbstract
Introduction
Toxoplasmosis a parasitic zoonosis of global distribution, responsible for disorders during gestation can cause fetal death or congenital anomalies.
Objective
To evaluate the knowledge of toxoplasmosis among pregnant and postpartum women treated at the University Hospital of the city of Rio Grande, Rio Grande do Sul, Brazil.
Methods
This was a cross-sectional study of 100 pregnant and postpartum women at the University Hospital. Participants answered a self-administered questionnaire and gave consent for data relating to serological examinations to be abstracted from their medical records.
Results
The proportion of women who received information about toxoplasmosis was higher among those who received care in the private health care system (52.9%) than among those cared for in the public health care system (25.0%). Only 55.7% of women reported having some knowledge about toxoplasmosis. Of these, 53.7% received information during the prenatal period. However, most participants were unable to answer questions about preventive measures and modes of infection. Of the 100 patients in the study, only 46 underwent serologic testing for toxoplasmosis, 65.2% of whom tested negative (IgG).
Conclusion
Findings from this study are relevant to the training of health professionals regarding toxoplasmosis education and prevention. Improved education for health care providers and patients can lead to earlier diagnoses and reductions in adverse outcomes.
Views114This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Summary
Original ArticlesEvaluation of Pregnant and Postpartum Women’s Knowledge about Toxoplasmosis in Rio Grande – RS, Brazil
Revista Brasileira de Ginecologia e Obstetrícia. 2016;38(11):538-544
11-01-2016Views114See moreAbstract
Introduction
Toxoplasmosis a parasitic zoonosis of global distribution, responsible for disorders during gestation can cause fetal death or congenital anomalies.
Objective
To evaluate the knowledge of toxoplasmosis among pregnant and postpartum women treated at the University Hospital of the city of Rio Grande, Rio Grande do Sul, Brazil.
Methods
This was a cross-sectional study of 100 pregnant and postpartum women at the University Hospital. Participants answered a self-administered questionnaire and gave consent for data relating to serological examinations to be abstracted from their medical records.
Results
The proportion of women who received information about toxoplasmosis was higher among those who received care in the private health care system (52.9%) than among those cared for in the public health care system (25.0%). Only 55.7% of women reported having some knowledge about toxoplasmosis. Of these, 53.7% received information during the prenatal period. However, most participants were unable to answer questions about preventive measures and modes of infection. Of the 100 patients in the study, only 46 underwent serologic testing for toxoplasmosis, 65.2% of whom tested negative (IgG).
Conclusion
Findings from this study are relevant to the training of health professionals regarding toxoplasmosis education and prevention. Improved education for health care providers and patients can lead to earlier diagnoses and reductions in adverse outcomes.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. -
Artigos Originais
Prevalence of positive sorology for HIV, hepatitis B, toxoplasmosis and rubella in pregnant women from the northwestern region of the state of Paraná
Revista Brasileira de Ginecologia e Obstetrícia. 2013;35(2):66-70
02-07-2013
Summary
Artigos OriginaisPrevalence of positive sorology for HIV, hepatitis B, toxoplasmosis and rubella in pregnant women from the northwestern region of the state of Paraná
Revista Brasileira de Ginecologia e Obstetrícia. 2013;35(2):66-70
02-07-2013DOI 10.1590/S0100-72032013000200005
Views69PURPOSE: To ascertain the seroprevalence of human immunodeficiency virus (HIV), hepatitis B (HBV), toxoplasmosis and rubella infections in pregnant women in northwestern Paraná. METHODS: We conducted a retrospective study based on the results of serological screening during prenatal care of 1,534 patients during the first half of 2010. We included only results from the first prenatal exam and with a simultaneous search for IgG and IgM antibodies to rubella and toxoplasmosis. Serology was performed by microparticle enzyme immunoassay (MEIA). Data were analyzed statistically by the χ² test, with the level of significance set at 5%. RESULTS: HIV positivity was 0.3%, positivity of HBV serology (HbsAg) was 0.5%, reactivity to IgM antibodies to Toxoplasma gondii was 1.1%, and reactivity to IgG antibodies was 59.0%. For rubella, no patient was positive for IgM, and IgG reactivity was 99.6%. Data analysis showed no statistical association between seroprevalence and patient age, except for the frequency of anti-T. gondii IgG, which was higher in the 30 to 44 year age group. CONCLUSION: The prevalence of these infectious diseases in pregnant women from northwestern Paraná is comparable to that observed in other regions of Brazil.
Key-words Hepatitis BHIV soropositivityInfectious diseases transmission verticalPregnant womanprenatal diagnosisRubellaToxoplasmosisSee moreViews69This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Summary
Artigos OriginaisPrevalence of positive sorology for HIV, hepatitis B, toxoplasmosis and rubella in pregnant women from the northwestern region of the state of Paraná
Revista Brasileira de Ginecologia e Obstetrícia. 2013;35(2):66-70
02-07-2013DOI 10.1590/S0100-72032013000200005
Views69PURPOSE: To ascertain the seroprevalence of human immunodeficiency virus (HIV), hepatitis B (HBV), toxoplasmosis and rubella infections in pregnant women in northwestern Paraná. METHODS: We conducted a retrospective study based on the results of serological screening during prenatal care of 1,534 patients during the first half of 2010. We included only results from the first prenatal exam and with a simultaneous search for IgG and IgM antibodies to rubella and toxoplasmosis. Serology was performed by microparticle enzyme immunoassay (MEIA). Data were analyzed statistically by the χ² test, with the level of significance set at 5%. RESULTS: HIV positivity was 0.3%, positivity of HBV serology (HbsAg) was 0.5%, reactivity to IgM antibodies to Toxoplasma gondii was 1.1%, and reactivity to IgG antibodies was 59.0%. For rubella, no patient was positive for IgM, and IgG reactivity was 99.6%. Data analysis showed no statistical association between seroprevalence and patient age, except for the frequency of anti-T. gondii IgG, which was higher in the 30 to 44 year age group. CONCLUSION: The prevalence of these infectious diseases in pregnant women from northwestern Paraná is comparable to that observed in other regions of Brazil.
Key-words Hepatitis BHIV soropositivityInfectious diseases transmission verticalPregnant womanprenatal diagnosisRubellaToxoplasmosisSee moreThis is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. -
Artigos Originais
Seroepidemiology of toxoplasmosis in pregnant women since the implementation of the Surveillance Program of Toxoplasmosis Acquired in Pregnancy and Congenital in the western region of Paraná, Brazil
Revista Brasileira de Ginecologia e Obstetrícia. 2012;34(2):63-68
03-15-2012
Summary
Artigos OriginaisSeroepidemiology of toxoplasmosis in pregnant women since the implementation of the Surveillance Program of Toxoplasmosis Acquired in Pregnancy and Congenital in the western region of Paraná, Brazil
Revista Brasileira de Ginecologia e Obstetrícia. 2012;34(2):63-68
03-15-2012DOI 10.1590/S0100-72032012000200004
Views91PURPOSE: To evaluate the susceptibility to toxoplasmosis in pregnant women in the public health service from two cities in the western region of Paraná, Brazil. METHODS: Four thousand twenty-two pregnant women were evaluated for anti-Toxoplasma gondii IgG and IgM by ELISA and MEIA. Seronegative pregnant women repeated the serology in the second and third trimester of pregnancy. Neonatal screening of 27 newborns was also performed in one of the cities to detect IgM anti- Toxoplasma gondii by fluorometry. All pregnant women answered an epidemiological questionnaire to analyze the factors associated with the risk of infection by Toxoplasma gondii. For statistical analysis, the presence of IgG anti-Toxoplasma gondii was considered as the dependent variable and the variables contained in the epidemiological questionnaire as the independent ones. RESULTS: The prevalence of anti-Toxoplasma gondii IgG in pregnant women was 59.8 and 60.6%. In one of the cities, the variables associated with the presence of IgG antibodies were low educational level and more than one pregnancy. There was no association with other factors studied such as consumption of raw or undercooked meat, consumption of raw vegetables, consumption of colonial salami, handling soil or sand, the presence of a home vegetable garden and cats in the household. In the other city there was no statistical association with the variables studied. No case of acute infection and no seroconversion were confirmed in either city. None of the infants evaluated were positive for toxoplasmosis. CONCLUSION: Toxoplasmosis is common in pregnant women attended by the public health service in the region studied and 40% of them are susceptible to the infection. These data reinforce the need to keep the screening program in these cities.
Key-words HIV seroprevalencePregnancy complications, parasiticToxoplasmaToxoplasmosisToxoplasmosis,congenitalSee moreViews91This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Summary
Artigos OriginaisSeroepidemiology of toxoplasmosis in pregnant women since the implementation of the Surveillance Program of Toxoplasmosis Acquired in Pregnancy and Congenital in the western region of Paraná, Brazil
Revista Brasileira de Ginecologia e Obstetrícia. 2012;34(2):63-68
03-15-2012DOI 10.1590/S0100-72032012000200004
Views91PURPOSE: To evaluate the susceptibility to toxoplasmosis in pregnant women in the public health service from two cities in the western region of Paraná, Brazil. METHODS: Four thousand twenty-two pregnant women were evaluated for anti-Toxoplasma gondii IgG and IgM by ELISA and MEIA. Seronegative pregnant women repeated the serology in the second and third trimester of pregnancy. Neonatal screening of 27 newborns was also performed in one of the cities to detect IgM anti- Toxoplasma gondii by fluorometry. All pregnant women answered an epidemiological questionnaire to analyze the factors associated with the risk of infection by Toxoplasma gondii. For statistical analysis, the presence of IgG anti-Toxoplasma gondii was considered as the dependent variable and the variables contained in the epidemiological questionnaire as the independent ones. RESULTS: The prevalence of anti-Toxoplasma gondii IgG in pregnant women was 59.8 and 60.6%. In one of the cities, the variables associated with the presence of IgG antibodies were low educational level and more than one pregnancy. There was no association with other factors studied such as consumption of raw or undercooked meat, consumption of raw vegetables, consumption of colonial salami, handling soil or sand, the presence of a home vegetable garden and cats in the household. In the other city there was no statistical association with the variables studied. No case of acute infection and no seroconversion were confirmed in either city. None of the infants evaluated were positive for toxoplasmosis. CONCLUSION: Toxoplasmosis is common in pregnant women attended by the public health service in the region studied and 40% of them are susceptible to the infection. These data reinforce the need to keep the screening program in these cities.
Key-words HIV seroprevalencePregnancy complications, parasiticToxoplasmaToxoplasmosisToxoplasmosis,congenitalSee moreThis is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. -
Artigos Originais
Prenatal screening for toxoplasmosis and factors associated with seropositivity of pregnant women in Goiânia, Goiás
Revista Brasileira de Ginecologia e Obstetrícia. 2011;33(2):93-98
07-08-2011
Summary
Artigos OriginaisPrenatal screening for toxoplasmosis and factors associated with seropositivity of pregnant women in Goiânia, Goiás
Revista Brasileira de Ginecologia e Obstetrícia. 2011;33(2):93-98
07-08-2011DOI 10.1590/S0100-72032011000200007
Views67PURPOSE: to estimate the prevalence and risk factors associated with seropositivity for Toxoplasma gondii in pregnant women. METHODS: a cross-sectional retrospective study based on the records of women screened for toxoplasmosis by the Pregnancy Protection Program in 2008, living in Goiânia (GO). These records were connected to records from the database of the National Information System on Live Births from the State of Goiás. The process occurred in three phases, with 10,316 records being paired for analysis, among the 12,846 initial records. The following variables were evaluated in this process: woman's name, age, date of birth, estimated date of delivery, date of infant birth and household information. Anti-Toxoplasma gondii antibodies were detected with the Q-Preven Toxo IgG and IgMin tests in dried blood samples collected on filter paper. The χ2 test and χ2 test for trend were used for data analysis, and the odds ratio (OR) was used to estimate the chance of association between exposure and outcome. RESULTS: the prevalence of infection was 67.7%, with 0.7% of the samples presenting anti-Toxoplasma gondii IgM and IgG reagents. Out of these, only three did not undergo confirmatory testing in venous blood. The median interval between the screening and the new collection of venous blood was of 12.5 days, and from screening to confirmatory test and avidity it was of 20 days. The variables associated with exposure were: age 20-30 years, OR=1.6 and >31 years, OR=1.8; brown skin color, OR=1.4, and black skin color, OR=1.6; and education of 8-11 years, OR=0.7, and >12 years of education, OR=0.6. CONCLUSION: a high prevalence of infection was estimated among the studied pregnant women. The associated factors that were found found should be considered during prenatal care, along with educational activities for the prevention of infection and assessment of serological status of seronegative pregnant women.
Key-words Cross-sectional studiesInfectious disease transmission, verticalPregnancyPrenatal careToxoplasmosisSee moreViews67This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Summary
Artigos OriginaisPrenatal screening for toxoplasmosis and factors associated with seropositivity of pregnant women in Goiânia, Goiás
Revista Brasileira de Ginecologia e Obstetrícia. 2011;33(2):93-98
07-08-2011DOI 10.1590/S0100-72032011000200007
Views67PURPOSE: to estimate the prevalence and risk factors associated with seropositivity for Toxoplasma gondii in pregnant women. METHODS: a cross-sectional retrospective study based on the records of women screened for toxoplasmosis by the Pregnancy Protection Program in 2008, living in Goiânia (GO). These records were connected to records from the database of the National Information System on Live Births from the State of Goiás. The process occurred in three phases, with 10,316 records being paired for analysis, among the 12,846 initial records. The following variables were evaluated in this process: woman's name, age, date of birth, estimated date of delivery, date of infant birth and household information. Anti-Toxoplasma gondii antibodies were detected with the Q-Preven Toxo IgG and IgMin tests in dried blood samples collected on filter paper. The χ2 test and χ2 test for trend were used for data analysis, and the odds ratio (OR) was used to estimate the chance of association between exposure and outcome. RESULTS: the prevalence of infection was 67.7%, with 0.7% of the samples presenting anti-Toxoplasma gondii IgM and IgG reagents. Out of these, only three did not undergo confirmatory testing in venous blood. The median interval between the screening and the new collection of venous blood was of 12.5 days, and from screening to confirmatory test and avidity it was of 20 days. The variables associated with exposure were: age 20-30 years, OR=1.6 and >31 years, OR=1.8; brown skin color, OR=1.4, and black skin color, OR=1.6; and education of 8-11 years, OR=0.7, and >12 years of education, OR=0.6. CONCLUSION: a high prevalence of infection was estimated among the studied pregnant women. The associated factors that were found found should be considered during prenatal care, along with educational activities for the prevention of infection and assessment of serological status of seronegative pregnant women.
Key-words Cross-sectional studiesInfectious disease transmission, verticalPregnancyPrenatal careToxoplasmosisSee moreThis is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Toxoplasmosis Archives - Revista Brasileira de Ginecologia e Obstetrícia
