in vitro fertilization Archives - Revista Brasileira de Ginecologia e Obstetrícia

  • Trabalhos Originais

    Evaluation of the Post-Biopsy Development of Bovine Embryos: Proposal of a Training Model

    Revista Brasileira de Ginecologia e Obstetrícia. 1999;21(9):533-538

    Summary

    Trabalhos Originais

    Evaluation of the Post-Biopsy Development of Bovine Embryos: Proposal of a Training Model

    Revista Brasileira de Ginecologia e Obstetrícia. 1999;21(9):533-538

    DOI 10.1590/S0100-72031999000900006

    Views3

    Purpose: to develop an animal model for the study of, and training in, bovine biopsies. Methods: cow ovaries were obtained from a slaughterhouse and transported to the laboratory where the oocytes were aspirated, maturated and submitted to in vitro fertilization. On the 5th day after fertilization, the embryos were biopsied, with the zona pellucida being opened with a cutting blade fitted to the light microscope. One or two blastomeres were removed from the embryos and left in coculture for three additional days. After this time, embryo development was evaluated in comparison to a control group by morphological study and cell counts using specific staining for nuclei. Results: forty of the 57 biopsied embryos reached the blastocyst stage (70.2%) and hatching was observed in 11 (27.5%). Forty-two blastocysts were obtained in the control group (73.7%) and 11 of them hatched (26.2%). Cell counts showed no significant differences between groups. Conclusions: we conclude that the proposed protocol is technically feasible and supplies a good number of embryos because of the easy technique for obtaining bovine oocytes, thus representing a method that could be adopted for training.

    See more
    Evaluation of the Post-Biopsy Development of Bovine Embryos: Proposal of a Training Model
  • Relato de Casos

    Twin pregnancy after thawing of morula embryos and blastocyst transfer: case report

    Revista Brasileira de Ginecologia e Obstetrícia. 1999;21(10):603-605

    Summary

    Relato de Casos

    Twin pregnancy after thawing of morula embryos and blastocyst transfer: case report

    Revista Brasileira de Ginecologia e Obstetrícia. 1999;21(10):603-605

    DOI 10.1590/S0100-72031999001000007

    Views0

    The cryopreservation of embryos in late developing stages seems to present satisfactory results. With the purpose of better testing the embryos' survival, they were cryopreserved in the morula or blastocyst stage, thawed and left in culture for 24 hours so that their natural evolution could be observed. Amongst the frozen 2 blastocysts and 5 morulas, 4 morulas survived the thawing process, being transferred as blastocysts 24 hours later. The transfer was performed in a young patient, second marriage of a ten-year vasectomized man and resulted in twin pregnancy. Thawing morula embryos and the in vitro observation of their development resumption until the blastocyst stage give us an additional parameter in the quality evaluation of the embryo and probably an improvement in pregnancy rates.

    See more
  • Trabalhos Originais

    Can polycystic ovary syndrome interfere with the outcome of in vitro fertilization?

    Revista Brasileira de Ginecologia e Obstetrícia. 2004;26(9):727-733

    Summary

    Trabalhos Originais

    Can polycystic ovary syndrome interfere with the outcome of in vitro fertilization?

    Revista Brasileira de Ginecologia e Obstetrícia. 2004;26(9):727-733

    DOI 10.1590/S0100-72032004000900009

    Views0

    PURPOSE: to evaluate the results of ovulation hyperinduction followed by in vitro fertilization (IVF) in women with polycystic ovary syndrome (POS), as compared to normal cycle women. METHODS: a controlled retrospective study conducted on 36 women with POS (POS group) and on 44 women with infertility due to mild male factor (control group), submitted to IVF from 1997 to 2003. Subject ages ranged from 18 to 36 years. Ovulation hyperinduction was obtained with recombinant follicle-stimulating hormone and a gonadotrophin-releasing hormone agonist. The analyzed variables were the follicles with a mean diameter of 14 to 17 mm and the follicles with diameters of 18 mm or above on the day of human chorionic gonadotrophin administration, percentage of follicles >18 mm, the number of retrieved oocytes, fertilization rate, cleavage rate, incidence of ovarian hyperstimulation syndrome (OHS), clinical pregnancy rate, and abortion rate. The variables were analyzed by the unpaired t test, Fisher exact test and Mann-Whitney test, with level of significance set at p<0.05. RESULTS: the POS group presented a larger number of retrieved follicles, most of them measuring 14 to 17 mm in diameter, compared to the control group (64.8 vs 53.9%), a lower fertilization rate (59.43 vs 79.57%) and a higher incidence of OHS (38.9 vs 9.1%). The number of retrieved oocytes, cleavage rates, pregnancy rates per embryo transfer, abortion rates and live born rates did not differ between groups. CONCLUSION: the success of IVF is impaired in women with POS due to their larger number of retrieved follicles of reduced diameter, reduced fertilization rate and high OHS rates.

    See more
  • Trabalhos Originais

    Bromocriptine method associated with ovarian stimulation in poor responder patients

    Revista Brasileira de Ginecologia e Obstetrícia. 2004;26(5):405-410

    Summary

    Trabalhos Originais

    Bromocriptine method associated with ovarian stimulation in poor responder patients

    Revista Brasileira de Ginecologia e Obstetrícia. 2004;26(5):405-410

    DOI 10.1590/S0100-72032004000500010

    Views0

    OBJECTIVE: to assess the ovarian response of poor responsive patients, submitted to the bromocriptine method. PACIENTS AND METHODS: a prospective clinical trial for the in vitro fertilization (IVF) program was performed in 10 poor responsive patients. Endocrinologically normal ovulatory women under 38 years old, who had previously failed in IVF due to poor response to ovarian stimulation with the traditional protocol, were submitted to the bromocriptine method in 12 cycles. They were given bromocriptine, a dopaminergic agonist, in the preceding cycle in order to stop the prolactin production. When the medication was removed at the beginning of the stimulation cycle, an elevation of seric prolactin by a rebound phenomenon was found. This optimized its seric concentration, improving the quality of oocytes and embryos. Serum prolactin and estradiol concentrations, number of follicles, number and quality of oocytes and cleaved embryos, fertilization and pregnancy rates were analyzed. RESULTS: there was a reduction in the dose of gonadotropin administered and in the duration of ovarian stimulation and an improvement in follicular recruitment, oocyte retrieval, embryo morphology, fertilization, and ongoing pregnancy rates. Fertilization rate was 77.7%, pregnancy rate was 44.4% and live baby rate was 25%. CONCLUSION: this study suggests that the bromocriptine method enhanced follicular recruitment and embryonic development, resulting in increased fertilization and pregnancy rates when compared with the traditional protocol for poor responsive patients. Studies with a large number of patients are necessary to confirm these results.

    See more
    Bromocriptine method associated with ovarian stimulation in poor responder patients
  • In vitro fertilization with low-cost programmed cycles – first outcome in a teaching hospital

    Revista Brasileira de Ginecologia e Obstetrícia. 2003;25(9):679-686

    Summary

    In vitro fertilization with low-cost programmed cycles – first outcome in a teaching hospital

    Revista Brasileira de Ginecologia e Obstetrícia. 2003;25(9):679-686

    DOI 10.1590/S0100-72032003000900010

    Views0
    PURPOSE: to evaluate the first in vitro fertilization results at a medical university using low-cost programmed cycles. METHODS: from May to December 2002, 66 programmed cycles of in vitro fertilization were carried out using norethisterone acetate, clomiphene citrate and human chorionic gonadotrophin (hCG). The ovarian follicle aspiration was guided by ultrasonography, 34 to 36 h […]
    See more
  • Trabalhos Originais

    Evaluation of the usefulness of the in vitro maturation technique of human oocyte and subsequent fertilization

    Revista Brasileira de Ginecologia e Obstetrícia. 2003;25(7):513-516

    Summary

    Trabalhos Originais

    Evaluation of the usefulness of the in vitro maturation technique of human oocyte and subsequent fertilization

    Revista Brasileira de Ginecologia e Obstetrícia. 2003;25(7):513-516

    DOI 10.1590/S0100-72032003000700008

    Views2

    PURPOSE: to evaluate the usefulness of the in vitro maturation technique of human oocyte and subsequent fertilization. METHODS: this is a prospective nonrandomized, descriptive study, carried out during the period of November 1999 to March 2001, with 20 cycles of in vitro fertilization of 15 patients with tubal infertility. All signed the written informed consent before the beginning of the study. The selected patients were at least 18 and at most 32 years of age, with only tubal infertility, and body mass index less than 25 kg/m². The patients received 300 UI of recombinant follicle stimulating hormone (FSH) by intramuscular injection at the second day of the cycle and additional doses of 150 IU at the fourth and sixth days of cycle. The oocyte retrieval was performed at the seventh day of the cycle. Those oocytes classified as immature were cultured in tissue culture medium 199 (TCM-199) with antibiotics, pyruvate, FSH, human chorionic gonadotropin (hCG) and serum (serum substitute supplement - Irvine Scientific®). After 48 h of culture, the oocytes that achieved metaphase II stage were inseminated, and the fertilized ones were transferred. RESULTS: one hundred and forty-four follicles were aspirated. There were 67 (46.5%) immature retrieved oocytes and 43 (64.2%) reached the metaphase II stage and were inseminated. Thirty fertilized oocytes and 25 embryos were transferred to 10 patients. There was one pregnancy with a baby born. CONCLUSION: we conclude that to mature human oocytes in vitro before in vitro fertilization is a procedure able to achieve pregnancy.

    See more
  • Trabalhos Originais

    Evaluation of the uterine cavity by sonohysterography in patients with implantation failure after in vitro fertilization

    Revista Brasileira de Ginecologia e Obstetrícia. 2003;25(7):519-523

    Summary

    Trabalhos Originais

    Evaluation of the uterine cavity by sonohysterography in patients with implantation failure after in vitro fertilization

    Revista Brasileira de Ginecologia e Obstetrícia. 2003;25(7):519-523

    DOI 10.1590/S0100-72032003000700009

    Views6

    PURPOSE: to analyze the accuracy of sonohysterography for the evaluation of the uterine cavity in patients with an implantation failure, at the first attempt of an in vitro fertilization cycle. METHODS: in a prospective double blind study, the authors analyzed patients previously submitted to at least one embryo transfer, who presented implantation failures. The patients were submitted to a sonohysterographic examination followed by a diagnostic hysteroscopic examination, carried out by different professionals each of whom was not aware of the results of the other. The results were recorded and only interpreted after the end of the trial. Sonohysterography was performed by the introduction of a urethral catheter 8 into the uterine cervix followed by infusion of physiological saline. The anechoic interface shown by the physiological saline can reveal abnormalities, like uterine polyps or submucosal myomas. Hysteroscopy was performed with a Karl Storz equipment, 4 mm 30º rigid telescope, and infusion of physiological saline for uterine cavity distention. RESULTS: twenty-eight of the 33 originally selected patients for this study were analyzed. Sonohysterography detected abnormalities in 8 patients, five with endometrial polyps (62.5%), two with endocervical polyps (25.0%), and one with submucosal myoma (12.5%). Hysteroscopy (gold standard) detected abnormalities in 7 patients, two with endometrial polyps (28.6%), two with cervical polyps (28.6%) and one with submucosal myoma (14.2%). Sonohysterography, when compared with diagnostic hysteroscopy, presented 71.4% sensibility, 85.7% specificity, 62.5% positive predictive value, and 90% negative predictive value of. CONCLUSION: due to its low positive predictive value, the authors suggest confirmation of the sonohysterography result by diagnostic hysteroscopy. Because sonohysterography presents a good level of specificity and a favorable low negative predictive value, the authors suggest that after a normal sonohysterography diagnostic hysteroscopy to evaluate the uterine cavity before in vitro fertlization is not necessary. This study leads to the conclusion that sonohysterography is a good screening method for the detection of polypoid lesions of the uterine cavity, which could be responsible for implantation failures in in vitro fertilization cycles.

    See more
  • Trabalhos Originais

    Comparison between three embryo scoring systems as predictive of the outcome of in vitro fertilization

    Revista Brasileira de Ginecologia e Obstetrícia. 2003;25(3):177-183

    Summary

    Trabalhos Originais

    Comparison between three embryo scoring systems as predictive of the outcome of in vitro fertilization

    Revista Brasileira de Ginecologia e Obstetrícia. 2003;25(3):177-183

    DOI 10.1590/S0100-72032003000300006

    Views2

    PURPOSE: to evaluate three embryo scoring systems specific for 3-day embryos and to correlate them with positive in vitro fertilization outcome. METHOD: retrospective study of the In Vitro Fertilization Program of the University Hospital, Faculty of Medicine of Ribeirão Preto, University of São Paulo. A total of 137 patients submitted to the transfer of 439 embryos were evaluated. The main outcomes measured were pregnancy and implantation rates. RESULTS: a significant difference in the three scoring systems was observed between pregnant (n=53) and non-pregnant (n=84) patients (p<0.0001). In the first embryo scoring system, in which cell number alone was used, higher pregnancy (70%) and implantation rates (42%) were observed when embryos with a mean blastomere number higher than 8 were transferred. Scoring system 2, based on a total four-point score (cleavage stage, blastomere number, fragmentation and symmetry), showed increased pregnancy (52.8%) and implantation rates (31%) for scores above 2. Scoring system 3, based on cell number and morphological criteria, also showed higher pregnancy and implantation rates with increasing average scores of the transferred embryos. CONCLUSION: the three scoring systems assessed in 3-day embryos were positively correlated with pregnancy and implantation rates.

    See more
    Comparison between three embryo scoring systems as predictive of the outcome of in vitro fertilization

Search

Search in:

Article type
abstract
book-review
brief-report
case-report
case-report -
correction
editorial
editorial -
letter
letter -
other
other -
rapid-communication
research-article
research-article -
review-article
review-article -
Section
Arigos Originais
Artigo de Revisão
Original Articles
Carta ao Editor
Carta ao Editor
Cartas
Case Report
Case Reports
Caso e Tratamento
Clinical Consensus Recommendation
Corrigendum
Editoriais
Editorial
Editorial
Equipamentos e Métodos
Errata
Erratas
Erratum
Febrasgo Position Statement
Febrasgo Statement
Febrasgo Statement Position
FIGO Statement
GUIDELINES
Integrative Review
Letter to Editor
Letter to Editor
Letter to the Editor
Letter to the Editor
Métodos e Técnicas
Nota do Editor
Nota Prévia
Original Article
Original Article/Contraception
Original Article/Infertility
Original Article/Obstetrics
Original Article/Oncology
Original Article/Sexual Violence/Pediatric and Adolescent Gynecology
Original Article/Teaching and Training
Original Articles
Original Articles
Relato de Caso
Relato de Casos
Relatos de Casos
Reply to the Letter to the Editor
Resposta dos Autores
Resumo De Tese
Resumo De Tese
Resumos de Tese
Resumos de Tese
Resumos de Teses
Resumos de Teses
Resumos dos Trabalhos Premiados no 50º Congresso Brasileiro de Ginecologia e Obstetrícia
Review
Review Article
Review Articles
Revisão
Revisão
Short Communication
Special Article
Systematic Review
Técnica e Equipamentos
Técnicas e Equipamentos
Técnicas e Métodos
Trabalhos Originais
Year / Volume
2024; v.46
2023; v.45
2022; v.44
2021; v.43
2020; v.42
2019; v.41
2018; v.40
2017; v.39
2016; v.38
2015; v.37
2014; v.36
2013; v.35
2012; v.34
2011; v.33
2010; v.32
2009; v.31
2008; v.30
2007; v.29
2006; v.28
2005; v.27
2004; v.26
2003; v.25
2002; v.24
2001; v.23
2000; v.22
1999; v.21
1998; v.20
ISSUE