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Revista Brasileira de Ginecologia e Obstetrícia. 1999;21(4):209-214
DOI 10.1590/S0100-72031999000400005
Purpose: to develop an experimental model in rats to study the interaction between hypertension and pregnancy. Methods: the present experiment was divided into 5 periods: adaptation (2 weeks), surgical procedures (1 week), hypertension development (6 weeks), mating and blood pressure stabilization (6 weeks), and gestational period (3 weeks). A total of 82 animals in reproductive age, weighing from 180 to 240 g, were used. They were randomly assigned to the 4 different groups (control, handled, nephrectomy and hypertension) and renal hypertension was produced by a controlled constriction of the main left renal artery, according to the technique described by Goldblatt, and contralateral nephrectomy (Goldblatt I - one kidney, one clip hypertension). They were studied at 15 precise moments. Afterwards, periodic blood pressure determinations were made by the tail plethysmographic method. Results: pregnancy caused a fall in blood pressure levels in the rat. Conclusion: the experimental model was adequate for the purposes of the study, since it proved to be efficient in producing hypertension.
Summary
Revista Brasileira de Ginecologia e Obstetrícia. 1999;21(4):209-214
DOI 10.1590/S0100-72031999000400005
Purpose: to develop an experimental model in rats to study the interaction between hypertension and pregnancy. Methods: the present experiment was divided into 5 periods: adaptation (2 weeks), surgical procedures (1 week), hypertension development (6 weeks), mating and blood pressure stabilization (6 weeks), and gestational period (3 weeks). A total of 82 animals in reproductive age, weighing from 180 to 240 g, were used. They were randomly assigned to the 4 different groups (control, handled, nephrectomy and hypertension) and renal hypertension was produced by a controlled constriction of the main left renal artery, according to the technique described by Goldblatt, and contralateral nephrectomy (Goldblatt I - one kidney, one clip hypertension). They were studied at 15 precise moments. Afterwards, periodic blood pressure determinations were made by the tail plethysmographic method. Results: pregnancy caused a fall in blood pressure levels in the rat. Conclusion: the experimental model was adequate for the purposes of the study, since it proved to be efficient in producing hypertension.
Summary
Revista Brasileira de Ginecologia e Obstetrícia. 2001;23(1):9-14
DOI 10.1590/S0100-72032001000100002
Purpose: to evaluate the effects of maternal diabetes on the fetal lung phospholipid profiles of rats with moderate and severe diabetes measuring lecithin (L), sphingomyelin (S), phosphatidyl-glycerol (PG), phosphatidyl-inositol (PI), and the relationships between L/S and PG/PI. Methods: fifty-four mature Wistar rats were submitted to experimental diabetes and pregnancy¹. Diabetes was induced by alloxan (42 mg/kg of weight, iv) and three groups were formed: control; moderate diabetes (MD), with glycemia levels between 120 and 200 mg/dl, and severe diabetes (SD), with glycemia levels higher than 200 mg/dl. On the 21st day, cesarian section was performed, and the fetal lungs were macerated and pooled. The phospholipids were measured by unidirectional thin-layer chromatography. Results: 1) the fetal lungs of the rats with moderate diabetes showed higher weight (0.159 g) and lower concentration of PG (3.0 µg/ml) and PI (3.4 µg/ml) than the controls, and the same relationship between L/S (2.2) and PG/PI (2.0). The fetal lungs of the rats with severe diabetes showed lower weight (0.145 g), the same values of L/S (1.9) and PG/PI (2.1), and lower PI (5.1 µg/ml) value than the controls. Conclusions: 1) the pulmonary maturity retardation in the pups of rats with moderate diabetes is explained by the higher pulmonary weight associated with lower concentration of PG and PI; 2) the pulmonary maturity acceleration in the pups of rats with severe diabetes is explained by the lower pulmonary weight associated with the same concentration of PG and PI.
Summary
Revista Brasileira de Ginecologia e Obstetrícia. 2001;23(1):9-14
DOI 10.1590/S0100-72032001000100002
Purpose: to evaluate the effects of maternal diabetes on the fetal lung phospholipid profiles of rats with moderate and severe diabetes measuring lecithin (L), sphingomyelin (S), phosphatidyl-glycerol (PG), phosphatidyl-inositol (PI), and the relationships between L/S and PG/PI. Methods: fifty-four mature Wistar rats were submitted to experimental diabetes and pregnancy¹. Diabetes was induced by alloxan (42 mg/kg of weight, iv) and three groups were formed: control; moderate diabetes (MD), with glycemia levels between 120 and 200 mg/dl, and severe diabetes (SD), with glycemia levels higher than 200 mg/dl. On the 21st day, cesarian section was performed, and the fetal lungs were macerated and pooled. The phospholipids were measured by unidirectional thin-layer chromatography. Results: 1) the fetal lungs of the rats with moderate diabetes showed higher weight (0.159 g) and lower concentration of PG (3.0 µg/ml) and PI (3.4 µg/ml) than the controls, and the same relationship between L/S (2.2) and PG/PI (2.0). The fetal lungs of the rats with severe diabetes showed lower weight (0.145 g), the same values of L/S (1.9) and PG/PI (2.1), and lower PI (5.1 µg/ml) value than the controls. Conclusions: 1) the pulmonary maturity retardation in the pups of rats with moderate diabetes is explained by the higher pulmonary weight associated with lower concentration of PG and PI; 2) the pulmonary maturity acceleration in the pups of rats with severe diabetes is explained by the lower pulmonary weight associated with the same concentration of PG and PI.
