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Original Article
Placental Sampling for Understanding Viral Infections – A Simplified Protocol for the COVID-19 Pandemic
- Guilherme de Moraes Nobrega
, - José Paulo Siqueira Guida ,
- Rodolfo Rosa Japecanga ,
- Arthur Antolini-Tavares ,
- Indira Mysorekar , [ ... ],
- Maria Laura Costa
07-30-2021
Summary
Original ArticlePlacental Sampling for Understanding Viral Infections – A Simplified Protocol for the COVID-19 Pandemic
Revista Brasileira de Ginecologia e Obstetrícia. 2021;43(5):377-383
07-30-2021- Guilherme de Moraes Nobrega ,
- José Paulo Siqueira Guida ,
- Rodolfo Rosa Japecanga ,
- Arthur Antolini-Tavares ,
- Indira Mysorekar ,
- Maria Laura Costa
Views120See moreAbstract
Objective
The coronavirus disease 2019 (COVID-19) is a pandemic viral disease, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The impact of the disease among the obstetric population remains unclear, and the study of the placenta can provide valuable information. Adequate sampling of the placental tissue can help characterize the pathways of viral infections.
Methods
A protocol of placental sampling is proposed, aiming at guaranteeing representativity of the placenta and describing the adequate conservation of samples and their integrity for future analysis. The protocol is presented in its complete and simplified versions, allowing its implementation in different complexity settings.
Results
Sampling with the minimum possible interval from childbirth is the key for adequate sampling and storage. This protocol has already been implemented during the Zika virus outbreak.
Conclusion
A protocol for adequate sampling and storage of placental tissue is fundamental for adequate evaluation of viral infections on the placenta. During the COVID-19 pandemic, implementation of this protocol may help to elucidate critical aspects of the SARS-CoV-2 infection.
Views120
This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Summary
Original ArticlePlacental Sampling for Understanding Viral Infections – A Simplified Protocol for the COVID-19 Pandemic
Revista Brasileira de Ginecologia e Obstetrícia. 2021;43(5):377-383
07-30-2021- Guilherme de Moraes Nobrega ,
- José Paulo Siqueira Guida ,
- Rodolfo Rosa Japecanga ,
- Arthur Antolini-Tavares ,
- Indira Mysorekar ,
- Maria Laura Costa
Views120See moreAbstract
Objective
The coronavirus disease 2019 (COVID-19) is a pandemic viral disease, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The impact of the disease among the obstetric population remains unclear, and the study of the placenta can provide valuable information. Adequate sampling of the placental tissue can help characterize the pathways of viral infections.
Methods
A protocol of placental sampling is proposed, aiming at guaranteeing representativity of the placenta and describing the adequate conservation of samples and their integrity for future analysis. The protocol is presented in its complete and simplified versions, allowing its implementation in different complexity settings.
Results
Sampling with the minimum possible interval from childbirth is the key for adequate sampling and storage. This protocol has already been implemented during the Zika virus outbreak.
Conclusion
A protocol for adequate sampling and storage of placental tissue is fundamental for adequate evaluation of viral infections on the placenta. During the COVID-19 pandemic, implementation of this protocol may help to elucidate critical aspects of the SARS-CoV-2 infection.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. - Guilherme de Moraes Nobrega
viral infections Archives - Revista Brasileira de Ginecologia e Obstetrícia
