Tibolone Archives - Revista Brasileira de Ginecologia e Obstetrícia
, Summary
Revista Brasileira de Ginecologia e Obstetrícia. 2019;41(7):449-453
, To analyze the effects of estrogen alone or in combination with progestogens and tibolone (TIB) on the expression of the extracellular matrix metalloproteinases 2 and 9 (MMP-2 and MMP-9), of perlecan, and of heparanase (HPSE) of the vascular walls of the carotid arteries.
A total of 30 250-day-old ovariectomized Wistar rats were orally treated for 5 weeks with: a) 1 mg/kg of estradiol benzoate (EB); b) EB + 0.2 mg/kg of medroxyprogesterone acetate (MPA); c) EB + 0.2mg/kg of norethisterone acetate (NETA); d) EB + 2 mg/kg of dydrogesterone (DI); e) 1 mg/kg of TIB; f) placebo (CTR). Following treatment, the expression of mRNA for MMP-2, MMP-9, and HPSE was analyzed by realtime polymerase chain-reaction (PCR), and the expression of MMP-2, of MMP-9, of tissue inhibitor of metalloproteinase 2 (TIMP-2), and of perlecan was quantified by immunohistochemistry in the carotid arteries.
The groups showed significant differences on mRNA HPSE expression (p = 0.048), which was higher in the EB, EB + MPA, and TIB groups. There was no statistically significant difference in mRNA MMP-2 or MMP-9 expression. The immunohistochemical expression of MMP-2, of TIMP-2, of MMP-9, of HPSE, and of perlecan showed no differences between groups.
Estradiol alone or associated with MPA and TIB treatment can increase mRNA HSPE expression of the walls of the carotid arteries in ovariectomized rats.
Summary
Revista Brasileira de Ginecologia e Obstetrícia. 2019;41(7):449-453
, To analyze the effects of estrogen alone or in combination with progestogens and tibolone (TIB) on the expression of the extracellular matrix metalloproteinases 2 and 9 (MMP-2 and MMP-9), of perlecan, and of heparanase (HPSE) of the vascular walls of the carotid arteries.
A total of 30 250-day-old ovariectomized Wistar rats were orally treated for 5 weeks with: a) 1 mg/kg of estradiol benzoate (EB); b) EB + 0.2 mg/kg of medroxyprogesterone acetate (MPA); c) EB + 0.2mg/kg of norethisterone acetate (NETA); d) EB + 2 mg/kg of dydrogesterone (DI); e) 1 mg/kg of TIB; f) placebo (CTR). Following treatment, the expression of mRNA for MMP-2, MMP-9, and HPSE was analyzed by realtime polymerase chain-reaction (PCR), and the expression of MMP-2, of MMP-9, of tissue inhibitor of metalloproteinase 2 (TIMP-2), and of perlecan was quantified by immunohistochemistry in the carotid arteries.
The groups showed significant differences on mRNA HPSE expression (p = 0.048), which was higher in the EB, EB + MPA, and TIB groups. There was no statistically significant difference in mRNA MMP-2 or MMP-9 expression. The immunohistochemical expression of MMP-2, of TIMP-2, of MMP-9, of HPSE, and of perlecan showed no differences between groups.
Estradiol alone or associated with MPA and TIB treatment can increase mRNA HSPE expression of the walls of the carotid arteries in ovariectomized rats.
Summary
Revista Brasileira de Ginecologia e Obstetrícia. 2015;37(5):233-240
DOI 10.1590/SO100-720320150005333
To assess the effect of tibolone on mammary tissue of castrated rats over 3
different periods of time.
Sixty virgin female Wistar rats were submitted to oophorectomy. Twenty-one days
after surgery, with hypoestrogenism confirmed, the experimental rats were randomly
assigned to six groups: Tibolone 1 (n=10) received tibolone 1 mg/day for 23 days,
tibolone 2 (n=10) for 59 days and tibolone 3 (n=10) for 118 days. The groups
control 1 (n=8), control 2 (n=7) and control 2 (n=10) received distilled water for
23, 59 and 118 days, respectively. After treatment, all six pairs of mammary
glands were removed and stained with hematoxylin and eosin (HE) for histological
analysis after euthanasia. The histological parameters evaluated were: epithelial
cell proliferation and secretory activity. The variables were analyzed
statistically, with the level of significance set at 0.05.
Histological changes were observed in 20/55 rats, mild epithelial hyperplasia in
7/55, moderate epithelial hyperplasia in 5/55, alveolar-nodular hyperplasia in
7/55, atypia without epithelial proliferation in 1/55, and no cases of severe
epithelial hyperplasia were found. Secretory activity was observed in 31/55 rats.
The secretory activity was significantly higher in the tibolone groups compared to
control at all the time points assessed (p=0,001). The histological changes were
did not show significance when the control and tibolone groups were compared. The
time of exposure to tibolone did not show significance when the three different
periods of evaluation were compared.
No relation between histological modification and tibolone treatment was verified
after short-, medium- and long-term treatment.
Summary
Revista Brasileira de Ginecologia e Obstetrícia. 2015;37(5):233-240
DOI 10.1590/SO100-720320150005333
To assess the effect of tibolone on mammary tissue of castrated rats over 3
different periods of time.
Sixty virgin female Wistar rats were submitted to oophorectomy. Twenty-one days
after surgery, with hypoestrogenism confirmed, the experimental rats were randomly
assigned to six groups: Tibolone 1 (n=10) received tibolone 1 mg/day for 23 days,
tibolone 2 (n=10) for 59 days and tibolone 3 (n=10) for 118 days. The groups
control 1 (n=8), control 2 (n=7) and control 2 (n=10) received distilled water for
23, 59 and 118 days, respectively. After treatment, all six pairs of mammary
glands were removed and stained with hematoxylin and eosin (HE) for histological
analysis after euthanasia. The histological parameters evaluated were: epithelial
cell proliferation and secretory activity. The variables were analyzed
statistically, with the level of significance set at 0.05.
Histological changes were observed in 20/55 rats, mild epithelial hyperplasia in
7/55, moderate epithelial hyperplasia in 5/55, alveolar-nodular hyperplasia in
7/55, atypia without epithelial proliferation in 1/55, and no cases of severe
epithelial hyperplasia were found. Secretory activity was observed in 31/55 rats.
The secretory activity was significantly higher in the tibolone groups compared to
control at all the time points assessed (p=0,001). The histological changes were
did not show significance when the control and tibolone groups were compared. The
time of exposure to tibolone did not show significance when the three different
periods of evaluation were compared.
No relation between histological modification and tibolone treatment was verified
after short-, medium- and long-term treatment.
