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Artigos Originais
Relationship between oocyte morphology and fertilization rate after ICSI
Revista Brasileira de Ginecologia e Obstetrícia. 2006;28(4):220-226
08-25-2006
Summary
Artigos OriginaisRelationship between oocyte morphology and fertilization rate after ICSI
Revista Brasileira de Ginecologia e Obstetrícia. 2006;28(4):220-226
08-25-2006DOI 10.1590/S0100-72032006000400003
Views103PURPOSE: to verify the possibility of identifying oocytes that would result in a higher fertilization rate. METHODS: retrospective analysis of the fertilization rate after ICSI of 957 oocytes in metaphase II according to three morphology parameters: cytoplasm inclusions, thickness of the perivitelline space, and fragmentation of the first polar body. Oocytes were obtained from 115 cycles performed among 107 women attended at the "Centro de Reprodução Humana de Campinas", from April to December of 2004. For the statistical analysis of differences in the fertilization rate between 'normal' oocytes and those presenting each alteration, the chi2 test was used with confidence levels of 5 and 10%. RESULTS: no significant difference in fertilization rate was observed regarding characteristics of the polar body or thickness of the perivitelline space. Fertilization rate among oocytes with perivitelline space with debris was 14 percentage points lower than among oocytes with absent space (p=0.055) and the rate among oocytes with granular cytoplasm was seven percentage points lower than among oocytes with normal cytoplasm (p<0.10>0.05). CONCLUSIONS: the morphological parameters of oocytes currently being evaluated do not allow us to clearly distinguish those that would lead to a higher fertilization rate and could be used in clinical practice.
Key-words Cytoplasm inclusionsFertilization in vitroFertilization rateOocytesPerivitelline spacePolar bodySee moreViews103
This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Summary
Artigos OriginaisRelationship between oocyte morphology and fertilization rate after ICSI
Revista Brasileira de Ginecologia e Obstetrícia. 2006;28(4):220-226
08-25-2006DOI 10.1590/S0100-72032006000400003
Views103PURPOSE: to verify the possibility of identifying oocytes that would result in a higher fertilization rate. METHODS: retrospective analysis of the fertilization rate after ICSI of 957 oocytes in metaphase II according to three morphology parameters: cytoplasm inclusions, thickness of the perivitelline space, and fragmentation of the first polar body. Oocytes were obtained from 115 cycles performed among 107 women attended at the "Centro de Reprodução Humana de Campinas", from April to December of 2004. For the statistical analysis of differences in the fertilization rate between 'normal' oocytes and those presenting each alteration, the chi2 test was used with confidence levels of 5 and 10%. RESULTS: no significant difference in fertilization rate was observed regarding characteristics of the polar body or thickness of the perivitelline space. Fertilization rate among oocytes with perivitelline space with debris was 14 percentage points lower than among oocytes with absent space (p=0.055) and the rate among oocytes with granular cytoplasm was seven percentage points lower than among oocytes with normal cytoplasm (p<0.10>0.05). CONCLUSIONS: the morphological parameters of oocytes currently being evaluated do not allow us to clearly distinguish those that would lead to a higher fertilization rate and could be used in clinical practice.
Key-words Cytoplasm inclusionsFertilization in vitroFertilization rateOocytesPerivitelline spacePolar bodySee moreThis is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Polar body Archives - Revista Brasileira de Ginecologia e Obstetrícia
