HPV Archives - Page 2 of 2 - Revista Brasileira de Ginecologia e Obstetrícia
Summary
Revista Brasileira de Ginecologia e Obstetrícia. 2004;26(4):277-287
DOI 10.1590/S0100-72032004000400003
OBJECTIVE: to compare the prevalence of DNA of human papillomavirus (HPV), in samples of normal endometrial tissue, and tissue with endometrial carcinoma of women submitted to surgical treatment (hysterectomy), or between endometrial carcinoma and benign disease, through the PCR technique. METHODS: this is an observational control-case study where 100 women (50 with endometrial carcinoma and 50 with normal endometrial tissue) were analyzed for the detection of HPV DNA in samples of endometrial tissue kept in paraffin blocks by the PCR technique. The cases of endometrial carcinoma with uncertain primary site of the lesion as well as the cases with previous or current history of pre-neoplasic lesions or carcinoma of the lower genital tract were excluded. Variables as age, smoking habit, endometrial trophism, squamous differentiation and degree of tumor differentiation were also evaluated. RESULTS: the estimated relative risk of the presence of HPV in the endometrial carcinoma and in the normal endometrial tissue was the same. HPV was detected in 8% of the cases of carcinoma and 10% in the normal endometrial tissue. In spite of HPV having been 3.5 times more detected in women with smoking habit in the group without carcinoma, there was no statistical difference. The presence of HPV was also not correlated with the women's age, endometrial trophism, squamous differentiation and degree of tumor differentiation. The HPV types 16 (5 cases) and 18 (4 cases) were the viruses most frequently found both in the normal endometrial tissue or in the tissue with carcinoma. No oncogenic low risk virus was detected in the samples. CONCLUSION: The same proportion of HPV is present in the endometrial tissue of women with endometrial cancer and with normal endometrium. It could not be demonstrated a possible correlation of DNA of HPV with the development of endometrial carcinoma.
Summary
Revista Brasileira de Ginecologia e Obstetrícia. 2004;26(4):277-287
DOI 10.1590/S0100-72032004000400003
OBJECTIVE: to compare the prevalence of DNA of human papillomavirus (HPV), in samples of normal endometrial tissue, and tissue with endometrial carcinoma of women submitted to surgical treatment (hysterectomy), or between endometrial carcinoma and benign disease, through the PCR technique. METHODS: this is an observational control-case study where 100 women (50 with endometrial carcinoma and 50 with normal endometrial tissue) were analyzed for the detection of HPV DNA in samples of endometrial tissue kept in paraffin blocks by the PCR technique. The cases of endometrial carcinoma with uncertain primary site of the lesion as well as the cases with previous or current history of pre-neoplasic lesions or carcinoma of the lower genital tract were excluded. Variables as age, smoking habit, endometrial trophism, squamous differentiation and degree of tumor differentiation were also evaluated. RESULTS: the estimated relative risk of the presence of HPV in the endometrial carcinoma and in the normal endometrial tissue was the same. HPV was detected in 8% of the cases of carcinoma and 10% in the normal endometrial tissue. In spite of HPV having been 3.5 times more detected in women with smoking habit in the group without carcinoma, there was no statistical difference. The presence of HPV was also not correlated with the women's age, endometrial trophism, squamous differentiation and degree of tumor differentiation. The HPV types 16 (5 cases) and 18 (4 cases) were the viruses most frequently found both in the normal endometrial tissue or in the tissue with carcinoma. No oncogenic low risk virus was detected in the samples. CONCLUSION: The same proportion of HPV is present in the endometrial tissue of women with endometrial cancer and with normal endometrium. It could not be demonstrated a possible correlation of DNA of HPV with the development of endometrial carcinoma.
Summary
Revista Brasileira de Ginecologia e Obstetrícia. 2004;26(1):59-64
DOI 10.1590/S0100-72032004000100009
PURPOSE: to carry out a molecular study (in situ hybridization) on patients who present intraepithelial lesions of the uterine cervix, and to assess the frequency and the physical state of the human papillomavirus (HPV). METHODS: histological sections of biopsies of the uterine cervix from 84 patients were evaluated by in situ hybridization, with a broad-spectrum probe, which allows the identification of the HPV types 6, 11, 16, 18, 31, 33, 35, 39, 42, 45, and 56 and with specific probes for HPV types 6, 11, 16, 18, 31, and 33. The physical patterns of HPV DNA found were: episomal, when the entire nucleus stains with biotin (brown); integrated - one or two brown points in the hybridized nucleus, or mixed, associating both patterns. Of the 84 patients evaluated, 31 (36.9%) had low-grade squamous intraepithelial lesions (LSIL), and 53 (63.1%) had high-grade squamous intraepithelial lesions (HSIL) on histological examination. Fisher's exact test was used for the statistical analysis. RESULTS: considering all the cases, 46 (54.7%) were positive for HPV DNA with the broad-spectrum probe. Regarding typing, HPV-16 was the most frequent in HSIL (12 cases - 22.6% - p<0.05). The frequencies of the other HPV types did not show statistically significant differences between the LSIL and HSIL cases. By physical condition assessment of the HPV DNA, the percentage of the episomal (most common in LSIL) and integrated patterns showed no significant differences between the two groups; the mixed HSIL type prevailed when compared to LSIL: 26.4 and 3.2%, respectively (p<0.01). The physical condition of the HPV DNA, integrated in the host cell, was more frequent in the most severe cases. CONCLUSIONS: HPV-16 was the most frequent in HSIL cases. The frequencies of the other HPV types did not show statistically significant differences between the LSIL and HSIL cases. The physical condition of the HPV DNA, integrated in the host cell, was more frequent in the more severe cases.
Summary
Revista Brasileira de Ginecologia e Obstetrícia. 2004;26(1):59-64
DOI 10.1590/S0100-72032004000100009
PURPOSE: to carry out a molecular study (in situ hybridization) on patients who present intraepithelial lesions of the uterine cervix, and to assess the frequency and the physical state of the human papillomavirus (HPV). METHODS: histological sections of biopsies of the uterine cervix from 84 patients were evaluated by in situ hybridization, with a broad-spectrum probe, which allows the identification of the HPV types 6, 11, 16, 18, 31, 33, 35, 39, 42, 45, and 56 and with specific probes for HPV types 6, 11, 16, 18, 31, and 33. The physical patterns of HPV DNA found were: episomal, when the entire nucleus stains with biotin (brown); integrated - one or two brown points in the hybridized nucleus, or mixed, associating both patterns. Of the 84 patients evaluated, 31 (36.9%) had low-grade squamous intraepithelial lesions (LSIL), and 53 (63.1%) had high-grade squamous intraepithelial lesions (HSIL) on histological examination. Fisher's exact test was used for the statistical analysis. RESULTS: considering all the cases, 46 (54.7%) were positive for HPV DNA with the broad-spectrum probe. Regarding typing, HPV-16 was the most frequent in HSIL (12 cases - 22.6% - p<0.05). The frequencies of the other HPV types did not show statistically significant differences between the LSIL and HSIL cases. By physical condition assessment of the HPV DNA, the percentage of the episomal (most common in LSIL) and integrated patterns showed no significant differences between the two groups; the mixed HSIL type prevailed when compared to LSIL: 26.4 and 3.2%, respectively (p<0.01). The physical condition of the HPV DNA, integrated in the host cell, was more frequent in the most severe cases. CONCLUSIONS: HPV-16 was the most frequent in HSIL cases. The frequencies of the other HPV types did not show statistically significant differences between the LSIL and HSIL cases. The physical condition of the HPV DNA, integrated in the host cell, was more frequent in the more severe cases.
Summary
Revista Brasileira de Ginecologia e Obstetrícia. 2002;24(5):315-320
DOI 10.1590/S0100-72032002000500005
Purpose: to study the association between the evaluation or not of the male partner and relapses in women treated for HPV lesions. Methods: a reconstituted cohort study with 144 women with HPV lesions and whose partners had been evaluated, compared with 288 women whose partners had not been examined, controlled regarding date of attendance, age, lesion degree and treatment, attended between July 1993 and March 2000. We evaluated similarity between the groups, the association between the evaluation or not of the partners or the diagnosis of lesions with the occurrence and relapse lesion degree in the women and the disease-free interval (DFI). Results: the groups were similar regarding the control variables. Relapses occurred in 9.0 % of the women whose partners had been evaluated and in 5.9% of those whose partners had not been evaluated (p=0.23). When lesions were diagnosed in the men, 12.5% of their partners had relapses, against 7.3% of the women whose partners had no lesions (p=0.23), but there was no correlation with the relapse lesion degree and DFI. When the men reported a time of monogamous conjugal relationship <12 months, we observed 14.9% relapses in women, against 6.2% for the women whose partners reported a longer time (p=0.08). Conclusions: evaluation of the man did not decrease the relapse risk of HPV lesions in his partner. The presence of lesions in the male partners did not correlate with the occurrence and relapse degree in women and DFI. This study does not support the hypothesis that nonevaluated men would be an important cause of relapses in their partners.
Summary
Revista Brasileira de Ginecologia e Obstetrícia. 2002;24(5):315-320
DOI 10.1590/S0100-72032002000500005
Purpose: to study the association between the evaluation or not of the male partner and relapses in women treated for HPV lesions. Methods: a reconstituted cohort study with 144 women with HPV lesions and whose partners had been evaluated, compared with 288 women whose partners had not been examined, controlled regarding date of attendance, age, lesion degree and treatment, attended between July 1993 and March 2000. We evaluated similarity between the groups, the association between the evaluation or not of the partners or the diagnosis of lesions with the occurrence and relapse lesion degree in the women and the disease-free interval (DFI). Results: the groups were similar regarding the control variables. Relapses occurred in 9.0 % of the women whose partners had been evaluated and in 5.9% of those whose partners had not been evaluated (p=0.23). When lesions were diagnosed in the men, 12.5% of their partners had relapses, against 7.3% of the women whose partners had no lesions (p=0.23), but there was no correlation with the relapse lesion degree and DFI. When the men reported a time of monogamous conjugal relationship <12 months, we observed 14.9% relapses in women, against 6.2% for the women whose partners reported a longer time (p=0.08). Conclusions: evaluation of the man did not decrease the relapse risk of HPV lesions in his partner. The presence of lesions in the male partners did not correlate with the occurrence and relapse degree in women and DFI. This study does not support the hypothesis that nonevaluated men would be an important cause of relapses in their partners.
