Group B Streptococcus Archives - Revista Brasileira de Ginecologia e Obstetrícia
Summary
Revista Brasileira de Ginecologia e Obstetrícia. 2005;27(10):575-579
DOI 10.1590/S0100-72032005001000002
PURPOSE: to verify the occurrence of colonization by Streptococcus agalactiae in pregnant women attended at the prenatal outpatient clinic of the Teaching Maternity Hospital of Rio de Janeiro University (UFRJ) and to evaluate the susceptibility of the isolates to antimicrobial agents. METHODS: a total of 167 pregnant women between the 32nd and 41st week of gestation, regardless of risk factors, attended at the antenatal clinic between February 2003 and February 2004, were evaluated. The vaginal/anal material, collected by the same swab, was inoculated in Todd-Hewitt broth to which nalidixic acid (15 µg/mL) and gentamicin (8 µg/mL) were added, with following subcultures onto sheep blood-agar. Identification was carried out observing colony morphology and beta-hemolysis type on blood-agar, catalase, cAMP, and serological tests. The antimicrobial susceptibility testing used agar diffusion and agar dilution methods. Statistical analysis was performed by the chi2 test with the level of significance set at p<0,05. RESULTS: the frequency of colonization was 19.2%, with no significant differences when age, number of gestations, number of abortions and the presence or absence of diabetes mellitus were compared (p>0.05). All 32 isolated strains were susceptible to penicillin, cefotaxime, ofloxacin, chloramphenicol, vancomycin and meropenem. Resistance to erythromycin and clindamycin was detected in 9.4 and 6.2% of the isolates, respectively. CONCLUSIONS: the relatively high incidence (19.2%) of colonization by S. agalactiae among the evaluated pregnant women and the recovery of antimicrobial resistant strains, especially those recommended in cases of penicillin allergy, emphasize the importance, for a correct prevention of neonatal infections, of detecting colonization at the end of pregnancy and evaluating antimicrobial susceptibility.
Summary
Revista Brasileira de Ginecologia e Obstetrícia. 2005;27(10):575-579
DOI 10.1590/S0100-72032005001000002
PURPOSE: to verify the occurrence of colonization by Streptococcus agalactiae in pregnant women attended at the prenatal outpatient clinic of the Teaching Maternity Hospital of Rio de Janeiro University (UFRJ) and to evaluate the susceptibility of the isolates to antimicrobial agents. METHODS: a total of 167 pregnant women between the 32nd and 41st week of gestation, regardless of risk factors, attended at the antenatal clinic between February 2003 and February 2004, were evaluated. The vaginal/anal material, collected by the same swab, was inoculated in Todd-Hewitt broth to which nalidixic acid (15 µg/mL) and gentamicin (8 µg/mL) were added, with following subcultures onto sheep blood-agar. Identification was carried out observing colony morphology and beta-hemolysis type on blood-agar, catalase, cAMP, and serological tests. The antimicrobial susceptibility testing used agar diffusion and agar dilution methods. Statistical analysis was performed by the chi2 test with the level of significance set at p<0,05. RESULTS: the frequency of colonization was 19.2%, with no significant differences when age, number of gestations, number of abortions and the presence or absence of diabetes mellitus were compared (p>0.05). All 32 isolated strains were susceptible to penicillin, cefotaxime, ofloxacin, chloramphenicol, vancomycin and meropenem. Resistance to erythromycin and clindamycin was detected in 9.4 and 6.2% of the isolates, respectively. CONCLUSIONS: the relatively high incidence (19.2%) of colonization by S. agalactiae among the evaluated pregnant women and the recovery of antimicrobial resistant strains, especially those recommended in cases of penicillin allergy, emphasize the importance, for a correct prevention of neonatal infections, of detecting colonization at the end of pregnancy and evaluating antimicrobial susceptibility.
Summary
Revista Brasileira de Ginecologia e Obstetrícia. 2004;26(7):543-549
DOI 10.1590/S0100-72032004000700006
PURPOSE: to study the prevalence of colonization by group B Streptococcus in pregnant women in the last three months of gestation, and to evaluate the association of colonization with demographic and clinical maternal variables. METHODS: from October 8, 2002 to March 26, 2003, a transversal study of prevalence of colonization by group B Streptococcus was carried out in 309 pregnant women in the last three months of gestation. Samples of vaginal and anorectal secretions were collected and were tested for presumptive identification of group B Streptococcus. The pregnant women were studied according to race, age, level of instruction, family income, number of gestations, gestational age, history of urinary tract infection during present pregnancy, premature rupture of membranes and duration of ruptured membranes, intrapartum fever, chorioamnionitis, meconium in the amniotic fluid, spontaneous or cesarean section delivery, postpartum fever, and postpartum endometritis. RESULTS: fourty-six pregnant women were diagnosed with group B Streptococcus: 26 of them (56.5%) had positive vaginal culture, 8 (17.4%) positive anorectal culture and 12 (26.5%) had both vaginal and anorectal positive cultures. None of the maternal variables were statistically significant with respect to group B Streptococcus colonization. The results were submitted to chi2 bivariate analysis and, when appropriate, Fisher's exact test. CONCLUSION: the prevalence rate of vaginal and anorectal colonization by group B Streptococcus in pregnant women in the last three months of gestation, in Londrina - Paraná, was 14.9%.
Summary
Revista Brasileira de Ginecologia e Obstetrícia. 2004;26(7):543-549
DOI 10.1590/S0100-72032004000700006
PURPOSE: to study the prevalence of colonization by group B Streptococcus in pregnant women in the last three months of gestation, and to evaluate the association of colonization with demographic and clinical maternal variables. METHODS: from October 8, 2002 to March 26, 2003, a transversal study of prevalence of colonization by group B Streptococcus was carried out in 309 pregnant women in the last three months of gestation. Samples of vaginal and anorectal secretions were collected and were tested for presumptive identification of group B Streptococcus. The pregnant women were studied according to race, age, level of instruction, family income, number of gestations, gestational age, history of urinary tract infection during present pregnancy, premature rupture of membranes and duration of ruptured membranes, intrapartum fever, chorioamnionitis, meconium in the amniotic fluid, spontaneous or cesarean section delivery, postpartum fever, and postpartum endometritis. RESULTS: fourty-six pregnant women were diagnosed with group B Streptococcus: 26 of them (56.5%) had positive vaginal culture, 8 (17.4%) positive anorectal culture and 12 (26.5%) had both vaginal and anorectal positive cultures. None of the maternal variables were statistically significant with respect to group B Streptococcus colonization. The results were submitted to chi2 bivariate analysis and, when appropriate, Fisher's exact test. CONCLUSION: the prevalence rate of vaginal and anorectal colonization by group B Streptococcus in pregnant women in the last three months of gestation, in Londrina - Paraná, was 14.9%.