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Original Article02-09-2022
Ovarian Tissue Culture to Preserve Fertility in Transgender Male Patients after Hormonal Treatment
- Alessandra Leal Bottini
, - Vânia Marisia Santos Fortes dos Reis ,
- Edison Capp ,
- Ilma Simoni Brum da Silva ,
- Lúcia Maria Kliemann , [ ... ],
- Helena von Eye Corleta
Abstract
Original ArticleOvarian Tissue Culture to Preserve Fertility in Transgender Male Patients after Hormonal Treatment
Revista Brasileira de Ginecologia e Obstetrícia. 2022;44(3):251-257
- Alessandra Leal Bottini ,
- Vânia Marisia Santos Fortes dos Reis ,
- Edison Capp ,
- Ilma Simoni Brum da Silva ,
- Lúcia Maria Kliemann ,
- Helena von Eye Corleta
Views178Abstract
Objective
To evaluate the reproductive and histological characteristics of fresh cultured ovarian tissue from transgender male patients.
Methods
An in vitro pilot study in which samples were collected during sex reassignment surgery for transgender male patients. The ovarian cortex was cut into fragments of 2 mm, 3mm, and 4 mm, and placed in a 96-well plate suitable for cultivation at days 0, 2, 4, 6, and 8, when the histology was analyzed.
Results
Stromal hyperplasia was observed in all samples, and it was not associated with the obtainment of primordial or primary follicles. Peripheral reduction in cell count was also a recurrent finding. Primordial and primary follicles were identified with a heterogeneous pattern in fragments from the same patient and from different patients, and follicles in more advanced stages of development (secondary and antral) were not found. There was an association between the diameter of the ovarian fragments and the identification of primary follicles (p=0.036). The number of days in culture was associated with histological signs of tissue damaging in the fragments (p=0.002). The total number of follicles identified in the samples with 2mm in diameter was significantly lower than in those that measured 4mm in diameter (p=0.031).
Conclusion
A diameter of 4mm is suitable for ovarian tissue culture with the benefit of ease of handling. Even after prolonged exposure to testosterone, the ovarian fragments presented primordial and primary follicles, maintaining viability throughout the days they were exposed to the culture. Freezing the ovarian cortex of transgender patients who will undergo surgery for gender reassignment would be an interesting option, in the future, for the preservation of fertility.
Key-words Fertility preservationgender reassignment surgeryOvarian reserveOvarytissue culture techniquesSee moreViews178
This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract
Original ArticleOvarian Tissue Culture to Preserve Fertility in Transgender Male Patients after Hormonal Treatment
Revista Brasileira de Ginecologia e Obstetrícia. 2022;44(3):251-257
- Alessandra Leal Bottini ,
- Vânia Marisia Santos Fortes dos Reis ,
- Edison Capp ,
- Ilma Simoni Brum da Silva ,
- Lúcia Maria Kliemann ,
- Helena von Eye Corleta
Views178Abstract
Objective
To evaluate the reproductive and histological characteristics of fresh cultured ovarian tissue from transgender male patients.
Methods
An in vitro pilot study in which samples were collected during sex reassignment surgery for transgender male patients. The ovarian cortex was cut into fragments of 2 mm, 3mm, and 4 mm, and placed in a 96-well plate suitable for cultivation at days 0, 2, 4, 6, and 8, when the histology was analyzed.
Results
Stromal hyperplasia was observed in all samples, and it was not associated with the obtainment of primordial or primary follicles. Peripheral reduction in cell count was also a recurrent finding. Primordial and primary follicles were identified with a heterogeneous pattern in fragments from the same patient and from different patients, and follicles in more advanced stages of development (secondary and antral) were not found. There was an association between the diameter of the ovarian fragments and the identification of primary follicles (p=0.036). The number of days in culture was associated with histological signs of tissue damaging in the fragments (p=0.002). The total number of follicles identified in the samples with 2mm in diameter was significantly lower than in those that measured 4mm in diameter (p=0.031).
Conclusion
A diameter of 4mm is suitable for ovarian tissue culture with the benefit of ease of handling. Even after prolonged exposure to testosterone, the ovarian fragments presented primordial and primary follicles, maintaining viability throughout the days they were exposed to the culture. Freezing the ovarian cortex of transgender patients who will undergo surgery for gender reassignment would be an interesting option, in the future, for the preservation of fertility.
Key-words Fertility preservationgender reassignment surgeryOvarian reserveOvarytissue culture techniquesSee moreThis is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. - Alessandra Leal Bottini
gender reassignment surgery Archives - Revista Brasileira de Ginecologia e Obstetrícia
