Embryo culture Archives - Revista Brasileira de Ginecologia e Obstetrícia
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Revista Brasileira de Ginecologia e Obstetrícia. 2022;44(11):1047-1051
, , , , , The use of granulocyte macrophage colony-stimulating factor (GM-CSF)-containing medium, which is a commercial medium that is used for cultivation of embryos in in vitro fertilization (IVF) treatments, has been suggested to increase the efficiency of this procedure in patients with previous multiple unsuccessful attempts. In this retrospective study, we analyzed GM-CSF-containing embryo culture media compared with traditional culture media in terms of development of embryos, pregnancy, and ongoing pregnancy success and live birth rates.
This is a prospective case control study conducted in a single center. A total of 131 unexplained infertility patients were included in the study. A cohort of 69 patients whose embryos were cultured in GM-CSF-containing medium and a control group of 62 age-matched patients whose embryos were cultured in conventional Sage One Step medium were included in the study. The major study outcomes were achievement of pregnancy and ongoing pregnancy rate at 12 weeks of gestation.
The pregnancy and ongoing pregnancy rates of the patients whose embryos were cultured in GM-CSF-containing medium were 39.13% and 36.23%, respectively. These were higher than the rates of the control group, which were 30.65% and 29.03%, respectively, although this difference was not statistically significant. In addition, the 5th-day embryo transfer percentage in the GM-CSF group was higher than in the control group (34.78% versus 27.4%).
The main findings of our study were that there was no difference between the GM-CSF-enhanced medium and the control group in terms of our major study outcomes. However, blastomere inequality rate and embryo fragmentation rates were lower in the GM-CSF group.
Summary
Revista Brasileira de Ginecologia e Obstetrícia. 2022;44(11):1047-1051
, , , , , The use of granulocyte macrophage colony-stimulating factor (GM-CSF)-containing medium, which is a commercial medium that is used for cultivation of embryos in in vitro fertilization (IVF) treatments, has been suggested to increase the efficiency of this procedure in patients with previous multiple unsuccessful attempts. In this retrospective study, we analyzed GM-CSF-containing embryo culture media compared with traditional culture media in terms of development of embryos, pregnancy, and ongoing pregnancy success and live birth rates.
This is a prospective case control study conducted in a single center. A total of 131 unexplained infertility patients were included in the study. A cohort of 69 patients whose embryos were cultured in GM-CSF-containing medium and a control group of 62 age-matched patients whose embryos were cultured in conventional Sage One Step medium were included in the study. The major study outcomes were achievement of pregnancy and ongoing pregnancy rate at 12 weeks of gestation.
The pregnancy and ongoing pregnancy rates of the patients whose embryos were cultured in GM-CSF-containing medium were 39.13% and 36.23%, respectively. These were higher than the rates of the control group, which were 30.65% and 29.03%, respectively, although this difference was not statistically significant. In addition, the 5th-day embryo transfer percentage in the GM-CSF group was higher than in the control group (34.78% versus 27.4%).
The main findings of our study were that there was no difference between the GM-CSF-enhanced medium and the control group in terms of our major study outcomes. However, blastomere inequality rate and embryo fragmentation rates were lower in the GM-CSF group.
Summary
Revista Brasileira de Ginecologia e Obstetrícia. 2001;23(9):589-595
DOI 10.1590/S0100-72032001000900007
Purpose: to compare the embryonic development obtained with two different culture methods (sequential medium or coculture in Vero cells). Methods: oocytes were recovered from 110 patients and submitted toin vitro fertilization. The embryos of half of the patients were co-cultured with Vero cells and the embryos of the other half were cultured in sequential G1:2/G2:2 medium for five days. The embryos were transferred on the 5th day after fertilization after morphological evaluation for the determination of blastula formation rate. Pregnancy was defined by ultrasonography and a fetal heartbeat was determined 13 weeks after transfer. Results: the expanded blastocyst rate found in our study was 15.9 and 14% with Vero cells and G1:2/G2:2, respectively. With Vero cells 36.0% of patients became pregnant and the implantation rate was 18.9%. When G1:2/G2:2 was used, the pregnancy and implantation rates were 28.9 and 14.9%, respectively. Only 17 patients had blastocysts after coculture in Vero cells, with a 76.5% pregnancy rate and a 63.5% implantation rate. When embryos were cultured in G1/G2, 21 patients presented blastocysts and the pregnancy and implantation rates were 57.1 and 76.0%, respectively. Conclusion: there was no significant difference in pregnancy or implantation rates between the 2 types of culture. When expanded blastocysts were transferred, the implantation and pregnancy rats increased with both culture types. In these patients, regardless of the type of culture used, a larger number of oocytes was obtained, suggesting that the implantation and pregnancy rates are affected not only by the culture conditions but also by the quality of the eggs, since "good responders" had better results.
Summary
Revista Brasileira de Ginecologia e Obstetrícia. 2001;23(9):589-595
DOI 10.1590/S0100-72032001000900007
Purpose: to compare the embryonic development obtained with two different culture methods (sequential medium or coculture in Vero cells). Methods: oocytes were recovered from 110 patients and submitted toin vitro fertilization. The embryos of half of the patients were co-cultured with Vero cells and the embryos of the other half were cultured in sequential G1:2/G2:2 medium for five days. The embryos were transferred on the 5th day after fertilization after morphological evaluation for the determination of blastula formation rate. Pregnancy was defined by ultrasonography and a fetal heartbeat was determined 13 weeks after transfer. Results: the expanded blastocyst rate found in our study was 15.9 and 14% with Vero cells and G1:2/G2:2, respectively. With Vero cells 36.0% of patients became pregnant and the implantation rate was 18.9%. When G1:2/G2:2 was used, the pregnancy and implantation rates were 28.9 and 14.9%, respectively. Only 17 patients had blastocysts after coculture in Vero cells, with a 76.5% pregnancy rate and a 63.5% implantation rate. When embryos were cultured in G1/G2, 21 patients presented blastocysts and the pregnancy and implantation rates were 57.1 and 76.0%, respectively. Conclusion: there was no significant difference in pregnancy or implantation rates between the 2 types of culture. When expanded blastocysts were transferred, the implantation and pregnancy rats increased with both culture types. In these patients, regardless of the type of culture used, a larger number of oocytes was obtained, suggesting that the implantation and pregnancy rates are affected not only by the culture conditions but also by the quality of the eggs, since "good responders" had better results.