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Summary
Revista Brasileira de Ginecologia e Obstetrícia. 2015;37(12):559-564
DOI 10.1590/SO100-720320150005462
To analyze the relation between the cytological findings and telomerase activity (TA).
Cervical samples were evaluated and classified according to the Bethesda System. Telomerase activity was measured total product generated values (TPG) using the TRAP assay (telomeric repeat amplification protocol); data were analyzed statistically using the χ2 test, with the level of significance set at p<0.05.
The study was conducted on 102 patients. Of these, 3.9% showed normal cytological findings, 8.8% showed cervicitis; 2% showed Atypical Squamous Cells of Undetermined Significance (ASCUS); 67.6% showed Low Grade Squamous Intraepithelial Lesion (LSIL); 11.8% showed High Grade Squamous Intraepithelial Lesion (H-SIL) and 5.9% showed Squamous Carcinoma. Among telomerase-positive samples, the TPG values were cervicitis Results show increased telomerase activity with increasing severity of lesion, supporting the association between TA and type of lesion.CONCLUSION
Summary
Revista Brasileira de Ginecologia e Obstetrícia. 2015;37(12):559-564
DOI 10.1590/SO100-720320150005462
To analyze the relation between the cytological findings and telomerase activity (TA).
Cervical samples were evaluated and classified according to the Bethesda System. Telomerase activity was measured total product generated values (TPG) using the TRAP assay (telomeric repeat amplification protocol); data were analyzed statistically using the χ2 test, with the level of significance set at p<0.05.
The study was conducted on 102 patients. Of these, 3.9% showed normal cytological findings, 8.8% showed cervicitis; 2% showed Atypical Squamous Cells of Undetermined Significance (ASCUS); 67.6% showed Low Grade Squamous Intraepithelial Lesion (LSIL); 11.8% showed High Grade Squamous Intraepithelial Lesion (H-SIL) and 5.9% showed Squamous Carcinoma. Among telomerase-positive samples, the TPG values were cervicitis Results show increased telomerase activity with increasing severity of lesion, supporting the association between TA and type of lesion.CONCLUSION
Summary
Revista Brasileira de Ginecologia e Obstetrícia. 2016;38(2):56-64
We studied the effects of loss of ovarian function (ovariectomy) onmuscle mass of gastrocnemius and themRNA levels of IGF-1, atrogin-1, MuRF-1, andmyostatin in an experimental model of rheumatoid arthritis in rats.
We randomly allocated 24 female Wistar rats (9 weeks, 195.3±17.4 grams) into four groups: control (CT-Sham; n = 6); rheumatoid arthritis (RA; n = 6); ovariectomy without rheumatoid arthritis (OV; n = 6); ovariectomy with rheumatoid arthritis (RAOV; n = 6). We performed the ovariectomy (OV and RAOV) or Sham (CTSham or RA) procedures at the same time, fifteen days before the rheumatoid arthritis induction. The RA and RAOV groups were immunized and then were injected with Met- BSA in the tibiotarsal joint. After 15 days of intra-articular injections the animals were euthanized. We evaluated the external manifestations of rheumatoid arthritis (perimeter joint) as well as animal weight, and food intake throughout the study. We also analyzed the cross-sectional areas (CSA) of gastrocnemius muscle fibers in 200 fibers (H&E method). In the gastrocnemius muscle, we analyzed mRNA expression by quantitative real time PCR followed by the Livak method (ΔΔCT).
The rheumatoid arthritis induced reduction in CSA of gastrocnemius muscle fibers. The RAOV group showed a lower CSA of gastrocnemius muscle fibers compared to RA and CT-Sham groups. Skeletal muscle IGF-1 mRNA increased in arthritics and ovariectomized rats. The increased IGF-1 mRNA was higher in OV groups than in the RA and RAOV groups. Antrogin-1 mRNA also increased in the gastrocnemius muscle of arthritic and ovariectomized rats. However, the increased atrogin-1 mRNA was higher in RAOV groups than in the RA and OV groups. Gastrocnemius muscle MuRF-1 mRNA increased in the OVand RAOVgroups, but not in the RA and Shamgroups. However, the RAOV group showed higher MuRF-1 mRNA than the OV group. The myostatin gene expression was similar in all groups.
Loss of ovarian function results in increased loss of skeletal musclerelated ubiquitin ligases atrogin-1 and MuRF-1 in arthritic rats.
Summary
Revista Brasileira de Ginecologia e Obstetrícia. 2016;38(2):56-64
We studied the effects of loss of ovarian function (ovariectomy) onmuscle mass of gastrocnemius and themRNA levels of IGF-1, atrogin-1, MuRF-1, andmyostatin in an experimental model of rheumatoid arthritis in rats.
We randomly allocated 24 female Wistar rats (9 weeks, 195.3±17.4 grams) into four groups: control (CT-Sham; n = 6); rheumatoid arthritis (RA; n = 6); ovariectomy without rheumatoid arthritis (OV; n = 6); ovariectomy with rheumatoid arthritis (RAOV; n = 6). We performed the ovariectomy (OV and RAOV) or Sham (CTSham or RA) procedures at the same time, fifteen days before the rheumatoid arthritis induction. The RA and RAOV groups were immunized and then were injected with Met- BSA in the tibiotarsal joint. After 15 days of intra-articular injections the animals were euthanized. We evaluated the external manifestations of rheumatoid arthritis (perimeter joint) as well as animal weight, and food intake throughout the study. We also analyzed the cross-sectional areas (CSA) of gastrocnemius muscle fibers in 200 fibers (H&E method). In the gastrocnemius muscle, we analyzed mRNA expression by quantitative real time PCR followed by the Livak method (ΔΔCT).
The rheumatoid arthritis induced reduction in CSA of gastrocnemius muscle fibers. The RAOV group showed a lower CSA of gastrocnemius muscle fibers compared to RA and CT-Sham groups. Skeletal muscle IGF-1 mRNA increased in arthritics and ovariectomized rats. The increased IGF-1 mRNA was higher in OV groups than in the RA and RAOV groups. Antrogin-1 mRNA also increased in the gastrocnemius muscle of arthritic and ovariectomized rats. However, the increased atrogin-1 mRNA was higher in RAOV groups than in the RA and OV groups. Gastrocnemius muscle MuRF-1 mRNA increased in the OVand RAOVgroups, but not in the RA and Shamgroups. However, the RAOV group showed higher MuRF-1 mRNA than the OV group. The myostatin gene expression was similar in all groups.
Loss of ovarian function results in increased loss of skeletal musclerelated ubiquitin ligases atrogin-1 and MuRF-1 in arthritic rats.
Summary
Revista Brasileira de Ginecologia e Obstetrícia. 2014;36(2):56-64
DOI 10.1590/S0100-72032014000200003
To evaluate the adequacy of the process of prenatal care according to the
parameters of the Program for the Humanization of Prenatal Care (PHPN) and of the
procedures provided by the Stork Network of Unified Health System (SUS) in the
microregion of Espirito Santo state, Brazil.
A cross-sectional study was conducted in 2012-2013 by interviewing and analyzing
the records of 742 women during the postpartum period and of their newborns in 7
hospitals in the region chosen for the research. The information was collected,
processed and analyzed by the χ2 and Fisher's exact test to determine
the difference in proportion between the criteria adopted by the PHPN and the
Stork Network and the place of residence, family income and type of coverage of
prenatal service. The level of significance was set at 5%.
The parameters showing the lowest adequacy rate were quick tests and repeated
exams, with frequencies around 10 and 30%, respectively, in addition to
educational activities (57.9%) and tetanus immunization (58.7%). In contrast, risk
management (92.6%) and the fasting plasma glucose test (91.3%) showed the best
results. Adequacy was 7.4% for the PHPN, 0.4% for the Stork Network, with respect
to the parameters of normal risk pregnancies, and 0 for high risk pregnancies.
There was a significant difference between puerperae according to housing location
regarding the execution of serology for syphilis (VDRL), anti-HIV and repeated
fasting glucose tests, and monthly income influenced the execution of blood
type/Rh factor tests, VDRL, hematocrit and anti-HIV test.
Prenatal care in the SUS proved to be inadequate regarding the procedures
required by the PHPN and Stork Network in the micro-region of a state in
southeastern Brazil, especially for women of lower income, PACS users and
residents of rural areas.
Summary
Revista Brasileira de Ginecologia e Obstetrícia. 2014;36(2):56-64
DOI 10.1590/S0100-72032014000200003
To evaluate the adequacy of the process of prenatal care according to the
parameters of the Program for the Humanization of Prenatal Care (PHPN) and of the
procedures provided by the Stork Network of Unified Health System (SUS) in the
microregion of Espirito Santo state, Brazil.
A cross-sectional study was conducted in 2012-2013 by interviewing and analyzing
the records of 742 women during the postpartum period and of their newborns in 7
hospitals in the region chosen for the research. The information was collected,
processed and analyzed by the χ2 and Fisher's exact test to determine
the difference in proportion between the criteria adopted by the PHPN and the
Stork Network and the place of residence, family income and type of coverage of
prenatal service. The level of significance was set at 5%.
The parameters showing the lowest adequacy rate were quick tests and repeated
exams, with frequencies around 10 and 30%, respectively, in addition to
educational activities (57.9%) and tetanus immunization (58.7%). In contrast, risk
management (92.6%) and the fasting plasma glucose test (91.3%) showed the best
results. Adequacy was 7.4% for the PHPN, 0.4% for the Stork Network, with respect
to the parameters of normal risk pregnancies, and 0 for high risk pregnancies.
There was a significant difference between puerperae according to housing location
regarding the execution of serology for syphilis (VDRL), anti-HIV and repeated
fasting glucose tests, and monthly income influenced the execution of blood
type/Rh factor tests, VDRL, hematocrit and anti-HIV test.
Prenatal care in the SUS proved to be inadequate regarding the procedures
required by the PHPN and Stork Network in the micro-region of a state in
southeastern Brazil, especially for women of lower income, PACS users and
residents of rural areas.
Summary
Revista Brasileira de Ginecologia e Obstetrícia. 2001;23(1):56-56
DOI 10.1590/S0100-72032001000100009
Summary
Revista Brasileira de Ginecologia e Obstetrícia. 2001;23(1):56-56
DOI 10.1590/S0100-72032001000100009
Summary
Revista Brasileira de Ginecologia e Obstetrícia. 2001;23(1):56-56
DOI 10.1590/S0100-72032001000100010
Summary
Revista Brasileira de Ginecologia e Obstetrícia. 2001;23(1):56-56
DOI 10.1590/S0100-72032001000100010
Summary
Revista Brasileira de Ginecologia e Obstetrícia. 2000;22(1):56-56
DOI 10.1590/S0100-72032000000100013
Summary
Revista Brasileira de Ginecologia e Obstetrícia. 2000;22(1):56-56
DOI 10.1590/S0100-72032000000100013
Summary
Revista Brasileira de Ginecologia e Obstetrícia. 2000;22(1):56-56
DOI 10.1590/S0100-72032000000100012
Summary
Revista Brasileira de Ginecologia e Obstetrícia. 2000;22(1):56-56
DOI 10.1590/S0100-72032000000100012
Summary
Revista Brasileira de Ginecologia e Obstetrícia. 2012;34(2):56-62
DOI 10.1590/S0100-72032012000200003
PURPOSE: To describe the characteristics of pregnancies complicated by maternal syphilis and fetal death. METHODS: Retrospective descriptive study performed by reviewing the medical records of 48 pregnant women with maternal syphilis and fetal death outcome admitted to Hospital Geral de Nova Iguaçu, Baixada Fluminense, State of Rio de Janeiro, during the period from 2005 to 2008. Birth weight >500 g and fetal death documented by Death Certificate were the inclusion criteria. The following aspects were analyzed: sociodemographic factors, reproductive history, aspects of the current pregnancy, prenatal care, Venereal Disease Research Laboratory (VDRL) testing, and other gestational conditions, in addition to syphilis. The fetal deaths were classified as maternal, placental or fetal. Percentage, mean, standard deviation (SD), maximum and minimum values were reported. RESULTS: The mean maternal age was 22.7 years (SD=0.9 years), and at least 50% of the patients had low educational level. At hospital admission, 68.8% of the subjects were in the third trimester, with a mean gestational age of 29.2 weeks (SD=0.5), and more than 50% were in labor. The vast majority of fetal deaths (93%) occurred before maternal hospitalization. Among the patients who received prenatal care (54.2%), 30.8% had no VDRL test, 30.8 and 15.4% had a reactive and non-reactive result, respectively, and none had more than one prenatal VDRL test. At the time of childbirth, most of the mothers (95.8%) carried out VDRL testing. Overall, the VDRL titers varied from 1:1 to 1:512, with predominant values >1:4 (91.7%). In 23% of cases other clinical conditions related to fetal death, in addition to syphilis, were found. CONCLUSIONS: The infection was the main clinically identified cause of fetal death in this patient series. Fetal death occurred during the preterm period and in the presence of high titers of maternal infection, suggesting recent syphilis infection.
Summary
Revista Brasileira de Ginecologia e Obstetrícia. 2012;34(2):56-62
DOI 10.1590/S0100-72032012000200003
PURPOSE: To describe the characteristics of pregnancies complicated by maternal syphilis and fetal death. METHODS: Retrospective descriptive study performed by reviewing the medical records of 48 pregnant women with maternal syphilis and fetal death outcome admitted to Hospital Geral de Nova Iguaçu, Baixada Fluminense, State of Rio de Janeiro, during the period from 2005 to 2008. Birth weight >500 g and fetal death documented by Death Certificate were the inclusion criteria. The following aspects were analyzed: sociodemographic factors, reproductive history, aspects of the current pregnancy, prenatal care, Venereal Disease Research Laboratory (VDRL) testing, and other gestational conditions, in addition to syphilis. The fetal deaths were classified as maternal, placental or fetal. Percentage, mean, standard deviation (SD), maximum and minimum values were reported. RESULTS: The mean maternal age was 22.7 years (SD=0.9 years), and at least 50% of the patients had low educational level. At hospital admission, 68.8% of the subjects were in the third trimester, with a mean gestational age of 29.2 weeks (SD=0.5), and more than 50% were in labor. The vast majority of fetal deaths (93%) occurred before maternal hospitalization. Among the patients who received prenatal care (54.2%), 30.8% had no VDRL test, 30.8 and 15.4% had a reactive and non-reactive result, respectively, and none had more than one prenatal VDRL test. At the time of childbirth, most of the mothers (95.8%) carried out VDRL testing. Overall, the VDRL titers varied from 1:1 to 1:512, with predominant values >1:4 (91.7%). In 23% of cases other clinical conditions related to fetal death, in addition to syphilis, were found. CONCLUSIONS: The infection was the main clinically identified cause of fetal death in this patient series. Fetal death occurred during the preterm period and in the presence of high titers of maternal infection, suggesting recent syphilis infection.