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  • Artigos Originais

    Functional activity of neutrophilic polymorphonuclear leukocytes in the first five days postpartum

    Rev Bras Ginecol Obstet. 2015;37(11):512-515

    Summary

    Artigos Originais

    Functional activity of neutrophilic polymorphonuclear leukocytes in the first five days postpartum

    Rev Bras Ginecol Obstet. 2015;37(11):512-515

    DOI 10.1590/SO100-720320150005434

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    PURPOSE:

    To assess the chemotactic activity and phagocytic response of neutrophilic polymorphonuclear leukocytes among women in the first five days postpartum.

    METHODS:

    A prospective, cross-sectional clinical-laboratory study was conducted. Data of 31 postpartum women during the first five days after vaginal delivery were compared with those of 24 healthy non-pregnant non-postpartum women matched for age. The inclusion criteria were postpartum, clinically and obstetrically healthy women; vaginal delivery, singleton pregnancy carried to term; non-hypertensive, hyperglycemic, allergic, malnourished or with autoimmune or neoplastic diseases; not having received vaccines or blood products in the last three months. The Control Group was chosen according to the same inclusion criteria but involving non-pregnant non-postpartum women. The chemotactic activity of neutrophilic polymorphonuclear leukocytes was assessed by determining the distance from directed migration to bacterial lipopolysaccharide, in three Boyden chamber assays. The phagocytic response was identified by assessing the Zymosan particles' ingestion in three assays carried out in Leighton tubes. The Student's t- test was used in the statistical analysis, adopting a 5% level of significance.

    RESULTS:

    The chemotactic activity of neutrophilic polymorphonuclear leukocytes from postpartum women in the presence of homologous (73.2±6.9) and autologous (78.6±13.9) sera showed a significant increase compared to the values observed in the Control Group (64.1±4.1 and 66.6±5.4). Both chemotactic response and phagocytosis ingestion phase of neutrophilic polymorphonuclear leukocytes were significantly increased (p<0.05) in postpartum women compared to healthy non-pregnant and non-postpartum women.

    CONCLUSION:

    There was an increase in the chemotactic activity and phagocytic response of neutrophilic polymorphonuclear leukocytes during the first five days after vaginal delivery in women.

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  • Original Article

    Lymphocyte and lymphocyte subset analyses in women with preeclampsia

    Rev Bras Ginecol Obstet. 2004;26(8):619-624

    Summary

    Original Article

    Lymphocyte and lymphocyte subset analyses in women with preeclampsia

    Rev Bras Ginecol Obstet. 2004;26(8):619-624

    DOI 10.1590/S0100-72032004000800005

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    PURPOSE: to analyze the relationship of the imune system with preeclamptic pregnancies, comparing lymphocyte and lymphocyte subset analyses of normal pregnant women to pregnancy-induced hypertensive women. To evaluate this immunological function, 40 pregnant women were studied at the "Irmandade da Santa Casa Hospital" of São Paulo: 20 women with normal pregnancies and 20 women with pregnancy-induced hypertension. From all these pregnant women peripheral blood samples were obtained and submitted to the following tests: lymphocyte and lymphocyte subsets analyses (CD4+, CD8+, CD4+/CD8 +CD3+, CD19+). Statistical analysis was performed by the Mann-Whitney test. RESULTS: the serum lymphocyte counts were decreased in women with preeclampsia (preeclamptic group 2295.10±1328.16; control group 3892.80±1430.85, p<0,05); and so were CD4+ (preeclamptic group 1188.80±625.61, control group; 1742.25±628.40, p<0.05); CD8+ (preeclamptic group: 774.00±371.31, control group 1175.70±517.72, p<0,05) and CD3+ (preeclamptic group 1958.65±983.78, control group 2916.95±1117.88, p<0,05). The other tests showed no significant differences between groups. CONCLUSION: the findings indicated a decreased number of lymphocytes, CD4+, CD8+ and CD3+ in preeclampsia.

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