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Rev Bras Ginecol Obstet. 2008;30(6):287-293
DOI 10.1590/S0100-72032008000600004
PURPOSE: to analyze the expression of matrix metalloproteinase-9 (MMP-9) and of vascular endothelial growth factor (EVGF) in a group of patients with primary breast cancer, and correlate them to one another and with other prognostic indicators. METHODS: transversal study that has analyzed the expression of MMP-9 and of VEGF in 88 consecutive cases of primary breast tumors. The samples were obtained from patients with primary breast cancer, submitted to surgical treatment in the Clinical Hospital of Porto Alegre of the Universidade Federal do Rio Grande do Sul, from January 2000 to December 2004. An immunohistochemical technique has been applied, using the avidin-biotin-peroxidase complex to evaluate the antigen immunoreactions in the tumors. The qualitative expression of proteins has been assessed through the observation of the brownish stain intensity of antibodies in the cytoplasm of malignant cells, when at least one of the tumoral cells presented clear and unequivocal staining with each of those markers. To determine the qualitative score (0=absent, 1=weak, 2=average and 3=strong), the stronger cytoplasmatic staining intensity on the glass slide has been taken into consideration, independently of the stained cells. The quantitative expression was determined by the average percentage of stained cells, observed in at least ten microscopic fields. The MMP-9 and VEGF final quantification expression has been done by the application of the HSCORE=Σ[(I+1)]xPC, where I and PC represent the staining intensity and the percentage of stained cells, respectively. RESULTS: MMP-9 and VEGF presented a significant correlation in the tumors studied. The final expression has shown a median score of 180 and 190, respectively. When MMP-9 and VEGF expression were compared with the variables "age", "tumoral diameter", "histological type", "histological grade", "axillary lymph node" and "vascular invasion", it was impossible to find any significant correlation. Compared to one another, MMP-0 and VEGF have presented a positive correlation (rho=0.23; p=0.03). The axillary lymph node positivity has presented a positive correlation with the larger tumoral diameter (2.7±1.1 cm; p<0.01) and with the presence of vascular invasion (84.1%; p<0.01). CONCLUSIONS: The present results do not show correlation between the MMP-9 and VEGF with the selected prognostic indicators, but shown a significant correlation between one another.
Summary
Rev Bras Ginecol Obstet. 2008;30(6):287-293
DOI 10.1590/S0100-72032008000600004
PURPOSE: to analyze the expression of matrix metalloproteinase-9 (MMP-9) and of vascular endothelial growth factor (EVGF) in a group of patients with primary breast cancer, and correlate them to one another and with other prognostic indicators. METHODS: transversal study that has analyzed the expression of MMP-9 and of VEGF in 88 consecutive cases of primary breast tumors. The samples were obtained from patients with primary breast cancer, submitted to surgical treatment in the Clinical Hospital of Porto Alegre of the Universidade Federal do Rio Grande do Sul, from January 2000 to December 2004. An immunohistochemical technique has been applied, using the avidin-biotin-peroxidase complex to evaluate the antigen immunoreactions in the tumors. The qualitative expression of proteins has been assessed through the observation of the brownish stain intensity of antibodies in the cytoplasm of malignant cells, when at least one of the tumoral cells presented clear and unequivocal staining with each of those markers. To determine the qualitative score (0=absent, 1=weak, 2=average and 3=strong), the stronger cytoplasmatic staining intensity on the glass slide has been taken into consideration, independently of the stained cells. The quantitative expression was determined by the average percentage of stained cells, observed in at least ten microscopic fields. The MMP-9 and VEGF final quantification expression has been done by the application of the HSCORE=Σ[(I+1)]xPC, where I and PC represent the staining intensity and the percentage of stained cells, respectively. RESULTS: MMP-9 and VEGF presented a significant correlation in the tumors studied. The final expression has shown a median score of 180 and 190, respectively. When MMP-9 and VEGF expression were compared with the variables "age", "tumoral diameter", "histological type", "histological grade", "axillary lymph node" and "vascular invasion", it was impossible to find any significant correlation. Compared to one another, MMP-0 and VEGF have presented a positive correlation (rho=0.23; p=0.03). The axillary lymph node positivity has presented a positive correlation with the larger tumoral diameter (2.7±1.1 cm; p<0.01) and with the presence of vascular invasion (84.1%; p<0.01). CONCLUSIONS: The present results do not show correlation between the MMP-9 and VEGF with the selected prognostic indicators, but shown a significant correlation between one another.
Summary
Rev Bras Ginecol Obstet. 2005;27(6):340-346
DOI 10.1590/S0100-72032005000600008
PURPOSE: a case-control study comparing two radiocolloids used in scintigraphy to map the sentinel lymph nodes (SLN) in breast cancer patients. METHODS: forty patients were prospectively enrolled between May 2002 and April 2004, after signing an informed consent form. In the present double-blind study, each patient was submitted twice to the same examination, a mammary scintigraphy, one with 99mTc-dextran 500 (dextran) and the other with 99mTc-phytate (phytate), on different days. A volume of 2 ml with 1-1.5 mCi of each radiopharmaceutical, in divided aliquots, was injected in the breast parenchyma in four points around in the tumor and the subcutaneous area superficial to the tumor. The image was obtained 2 h after the injection, using a gamma camera with high-resolution collimator. The lymph nodes were identified by anterior and lateral static scintigraphic images. Statistical analysis was done with the use of McNemar and Z tests. RESULTS: in the analysis of the 40 patients, we had 15 pairs with positive identical images, 4 pairs with negative images and 21 pairs with inconsistent images, either because one of them was negative, or because the SLN numbers were different. When the protocol was opened, we found 35 and 27 positive images and 5 and 13 negative images for dextran and phytate treatment groups, respectively. Among the negative images, 4 were shared by both groups. The McNemar test, used for the statistical analysis, showed p=0.026, odds ratio (OR) = 0.11 with 95% CI 0.01 < OR < 0.85. The accuracy, evaluated by the success ratio of the SLN mapping, was 67.5% for phytate and 87.5% for dextran, with p=0.032. Analysis of variance of the SLN number in lymphoscintigraphy images showed p=0.008. CONCLUSION: these results recommend the use of dextran instead of phytate for the SLN study of breast carcinoma by scintigraphy, when the same methodology is being used.
Summary
Rev Bras Ginecol Obstet. 2005;27(6):340-346
DOI 10.1590/S0100-72032005000600008
PURPOSE: a case-control study comparing two radiocolloids used in scintigraphy to map the sentinel lymph nodes (SLN) in breast cancer patients. METHODS: forty patients were prospectively enrolled between May 2002 and April 2004, after signing an informed consent form. In the present double-blind study, each patient was submitted twice to the same examination, a mammary scintigraphy, one with 99mTc-dextran 500 (dextran) and the other with 99mTc-phytate (phytate), on different days. A volume of 2 ml with 1-1.5 mCi of each radiopharmaceutical, in divided aliquots, was injected in the breast parenchyma in four points around in the tumor and the subcutaneous area superficial to the tumor. The image was obtained 2 h after the injection, using a gamma camera with high-resolution collimator. The lymph nodes were identified by anterior and lateral static scintigraphic images. Statistical analysis was done with the use of McNemar and Z tests. RESULTS: in the analysis of the 40 patients, we had 15 pairs with positive identical images, 4 pairs with negative images and 21 pairs with inconsistent images, either because one of them was negative, or because the SLN numbers were different. When the protocol was opened, we found 35 and 27 positive images and 5 and 13 negative images for dextran and phytate treatment groups, respectively. Among the negative images, 4 were shared by both groups. The McNemar test, used for the statistical analysis, showed p=0.026, odds ratio (OR) = 0.11 with 95% CI 0.01 < OR < 0.85. The accuracy, evaluated by the success ratio of the SLN mapping, was 67.5% for phytate and 87.5% for dextran, with p=0.032. Analysis of variance of the SLN number in lymphoscintigraphy images showed p=0.008. CONCLUSION: these results recommend the use of dextran instead of phytate for the SLN study of breast carcinoma by scintigraphy, when the same methodology is being used.
Summary
Rev Bras Ginecol Obstet. 2000;22(1):43-48
DOI 10.1590/S0100-72032000000100008
Purpose: to analyze the prevalence of genuine urinary incontinence (GUI) recurrence, after at least two years of follow-up, in different surgical techniques used for its correction. Patients and Methods: fifty-five patients with diagnosis of GUI, submitted to surgery for its repair at the Serviço de Ginecologia e Obstetrícia do Hospital de Clínicas de Porto Alegre from 1992 to 1996 and whose post-surgical follow-up was superior to 2 years were divided into three groups according to the surgical approach: Kelly-Kennedy (n = 24), Burch (n = 23) and Marshall-Marchetti-Krantz (n = 8). Results: there were no differences regarding recurrence rate, age at surgery and at recurrence time, estrogen therapy, number of pregnancies and vaginal delivery (p>0.05). Although posterior perineoplasty was more prevalent in the Kelly-Kennedy group, it did not influence the recurrence rate. The group submitted to the Burch approach had more years of menopause at the time of surgery. Conclusion: the recurrence rates of urinary incontinence comparing the three different techniques (Kelly-Kennedy, Burch and Marshall-Marchetti-Krantz) were, respectively, 29.2, 39.1 and 50%, which did not differ statistically. Considering the potential confusional bias for urinary stress incontinence, they did not differ among the groups. Nevertheless, we noticed that all women who had previous surgery presented recurrence of incontinence.
Summary
Rev Bras Ginecol Obstet. 2000;22(1):43-48
DOI 10.1590/S0100-72032000000100008
Purpose: to analyze the prevalence of genuine urinary incontinence (GUI) recurrence, after at least two years of follow-up, in different surgical techniques used for its correction. Patients and Methods: fifty-five patients with diagnosis of GUI, submitted to surgery for its repair at the Serviço de Ginecologia e Obstetrícia do Hospital de Clínicas de Porto Alegre from 1992 to 1996 and whose post-surgical follow-up was superior to 2 years were divided into three groups according to the surgical approach: Kelly-Kennedy (n = 24), Burch (n = 23) and Marshall-Marchetti-Krantz (n = 8). Results: there were no differences regarding recurrence rate, age at surgery and at recurrence time, estrogen therapy, number of pregnancies and vaginal delivery (p>0.05). Although posterior perineoplasty was more prevalent in the Kelly-Kennedy group, it did not influence the recurrence rate. The group submitted to the Burch approach had more years of menopause at the time of surgery. Conclusion: the recurrence rates of urinary incontinence comparing the three different techniques (Kelly-Kennedy, Burch and Marshall-Marchetti-Krantz) were, respectively, 29.2, 39.1 and 50%, which did not differ statistically. Considering the potential confusional bias for urinary stress incontinence, they did not differ among the groups. Nevertheless, we noticed that all women who had previous surgery presented recurrence of incontinence.
Summary
Rev Bras Ginecol Obstet. 2002;24(7):479-484
DOI 10.1590/S0100-72032002000700008
Purpose: to evaluate the lymphoscintigraphy, the hand-held probe and the vital blue dye to identify the sentinel lymph node (SLN) in breast carcinoma and to establish sensitivity, negative predictive value (NPV) and overall accuracy of the SLN detection. Methods: eighty-eight consecutive attended patients, with clinically negative axillary lymph nodes were enrolled for this study using the technique of mapping with 99m technetium dextran for scintigraphic images before surgery. In the operating room, five minutes before axillary incision, we injected 2 mL of 2.5% Bleu Patente V Sodique around the tumor and intradermally. Then the gamma probe helped to find out the hot spot where the SLN was supposed to be. The pathologic results of SLN were obtained by the standard technique with hematoxylin and eosin staining in seventy-seven patients. Results: scintigraphy was conclusive for lymphatic basins in 62.1% of 58 cases, concerning SLN identification. In these conclusive patients, there were 9 in which 2 nodes appeared simultaneously without lymph vessel delineation. Using the gamma probe, at least one axillary SLN site could be found preoperatively in 45 cases (84.9%), and intraoperatively, associated with blue dye, in all 53 patients. In 32 of 35 patients only vital blue dye was used with success. Concerning the groups "day" and "other day" (when biopsy was performed on the same day or the other day, after the radiolabeled injection), therefore different regarding time and gamma counts, both achieved the same results regarding SLN detection. Forty-one patients had positive axillary lymph nodes and only two had false negative SLN, giving sensitivity of 95.3%, a NPV of 95.5% and overall accuracy of 97.7%. Conclusions: the lymph node mapping with the use of technetium and patent blue V, alone or associated, is feasible. The time since the injection of technetium up to surgery varied from 3 to 17 h. The pathologic findings confirmed that the biopsied lymph node was the true sentinel node in 97.6% of the cases and validated the SLN mapping, and this should lead to an avoidance of complete axillary dissection as a routine procedure in patients with negative SLN.
Summary
Rev Bras Ginecol Obstet. 2002;24(7):479-484
DOI 10.1590/S0100-72032002000700008
Purpose: to evaluate the lymphoscintigraphy, the hand-held probe and the vital blue dye to identify the sentinel lymph node (SLN) in breast carcinoma and to establish sensitivity, negative predictive value (NPV) and overall accuracy of the SLN detection. Methods: eighty-eight consecutive attended patients, with clinically negative axillary lymph nodes were enrolled for this study using the technique of mapping with 99m technetium dextran for scintigraphic images before surgery. In the operating room, five minutes before axillary incision, we injected 2 mL of 2.5% Bleu Patente V Sodique around the tumor and intradermally. Then the gamma probe helped to find out the hot spot where the SLN was supposed to be. The pathologic results of SLN were obtained by the standard technique with hematoxylin and eosin staining in seventy-seven patients. Results: scintigraphy was conclusive for lymphatic basins in 62.1% of 58 cases, concerning SLN identification. In these conclusive patients, there were 9 in which 2 nodes appeared simultaneously without lymph vessel delineation. Using the gamma probe, at least one axillary SLN site could be found preoperatively in 45 cases (84.9%), and intraoperatively, associated with blue dye, in all 53 patients. In 32 of 35 patients only vital blue dye was used with success. Concerning the groups "day" and "other day" (when biopsy was performed on the same day or the other day, after the radiolabeled injection), therefore different regarding time and gamma counts, both achieved the same results regarding SLN detection. Forty-one patients had positive axillary lymph nodes and only two had false negative SLN, giving sensitivity of 95.3%, a NPV of 95.5% and overall accuracy of 97.7%. Conclusions: the lymph node mapping with the use of technetium and patent blue V, alone or associated, is feasible. The time since the injection of technetium up to surgery varied from 3 to 17 h. The pathologic findings confirmed that the biopsied lymph node was the true sentinel node in 97.6% of the cases and validated the SLN mapping, and this should lead to an avoidance of complete axillary dissection as a routine procedure in patients with negative SLN.
Summary
Rev Bras Ginecol Obstet. 2010;32(10):486-490
DOI 10.1590/S0100-72032010001000003
PURPOSE: to determine the efficacy of intraoperative injection of Dextran-500-99m-technetium (Tc) for the identification of the sentinel lymph node (SLN) in breast cancer and analyze time to label the SLN in the axillary region. METHODS: a prospective study between April 2008 and June 2009, which included 74 sentinel lymph node biopsies (SLNB) in patients with breast cancer in stages T1N0 and T2N0. After induction of anesthesia, 0.5 to 1.5 mCi of Dextran-500-99m-Tc filtered 0.22 µm in a volume of 5 mL was injected intraoperative using the subareolar technique for SLNB. After labeling with the radioisotope, 2 mL of patent blue was injected. The time elapsed between injection and the axillary hot spot, the in vivo and ex vivo counts of the hottest nodes, the background count, and the number of SLN identified were documented. Data were analyzed using descriptive statistics with SPSS program, version 18. RESULTS: we identified the SLN in 100% of cases. The rate of SLN identification with the probe was 98% (73/74 cases). In one case (1.35%) the SLN was labeled only with the blue dye. The mean dose of radioisotope injected was 0.97±0.22 mCi. The average time to label the SLN was 10.7 minutes (±5.7 min). We identified on average of 1.66 SLN labeled with the radioisotope. CONCLUSION: the procedure for SLN identification with an intraoperative injection of the radioisotope is oncologically safe and comfortable for the patient, providing agility to the surgical team.
Summary
Rev Bras Ginecol Obstet. 2010;32(10):486-490
DOI 10.1590/S0100-72032010001000003
PURPOSE: to determine the efficacy of intraoperative injection of Dextran-500-99m-technetium (Tc) for the identification of the sentinel lymph node (SLN) in breast cancer and analyze time to label the SLN in the axillary region. METHODS: a prospective study between April 2008 and June 2009, which included 74 sentinel lymph node biopsies (SLNB) in patients with breast cancer in stages T1N0 and T2N0. After induction of anesthesia, 0.5 to 1.5 mCi of Dextran-500-99m-Tc filtered 0.22 µm in a volume of 5 mL was injected intraoperative using the subareolar technique for SLNB. After labeling with the radioisotope, 2 mL of patent blue was injected. The time elapsed between injection and the axillary hot spot, the in vivo and ex vivo counts of the hottest nodes, the background count, and the number of SLN identified were documented. Data were analyzed using descriptive statistics with SPSS program, version 18. RESULTS: we identified the SLN in 100% of cases. The rate of SLN identification with the probe was 98% (73/74 cases). In one case (1.35%) the SLN was labeled only with the blue dye. The mean dose of radioisotope injected was 0.97±0.22 mCi. The average time to label the SLN was 10.7 minutes (±5.7 min). We identified on average of 1.66 SLN labeled with the radioisotope. CONCLUSION: the procedure for SLN identification with an intraoperative injection of the radioisotope is oncologically safe and comfortable for the patient, providing agility to the surgical team.
Summary
Rev Bras Ginecol Obstet. 1998;20(10):557-561
DOI 10.1590/S0100-72031998001000003
Purpose: the authors report their experience with necrotizing fasciitis (NF) cases which occurred in the Gynecology and Obstetrics Service of the Hospital de Clínicas de Porto Alegre, assessing the frequency of NF and analyzing the association between NF and certain risk factors cited in the literature. Methods: a retrospective study of patients a with diagnosis of necrotizing fasciitis at the Hospital de Clínicas de Porto Alegre from January 1990 to December 1997. Results: two post-cesarean section and one post-surgical (because of ectopic pregnancy) NF cases were found. None of the patients presented clinical complications nor NF risk factors and all surgeries were urgent. The NF frequency in this study was 2.6/10.000 cesarians and mortality was zero. Discussion: NF is a clinical syndrome which does not occur very often but is associated with high morbidity and mortality. This disease involves the surgical wound and the fascial plans. Fast handling and early and intensive treatment bring about good results and decrease in the mortality rate.
Summary
Rev Bras Ginecol Obstet. 1998;20(10):557-561
DOI 10.1590/S0100-72031998001000003
Purpose: the authors report their experience with necrotizing fasciitis (NF) cases which occurred in the Gynecology and Obstetrics Service of the Hospital de Clínicas de Porto Alegre, assessing the frequency of NF and analyzing the association between NF and certain risk factors cited in the literature. Methods: a retrospective study of patients a with diagnosis of necrotizing fasciitis at the Hospital de Clínicas de Porto Alegre from January 1990 to December 1997. Results: two post-cesarean section and one post-surgical (because of ectopic pregnancy) NF cases were found. None of the patients presented clinical complications nor NF risk factors and all surgeries were urgent. The NF frequency in this study was 2.6/10.000 cesarians and mortality was zero. Discussion: NF is a clinical syndrome which does not occur very often but is associated with high morbidity and mortality. This disease involves the surgical wound and the fascial plans. Fast handling and early and intensive treatment bring about good results and decrease in the mortality rate.